Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, California, USA.
Fertil Steril. 2010 Jan;93(1):257-63. doi: 10.1016/j.fertnstert.2008.09.074. Epub 2008 Nov 19.
To determine the direct effects of cigarette smoke (CS) on human endometrial surface epithelial cell line proliferation.
In vitro study using HES cells and primary human endometrial cells.
University-based academic center.
PATIENT(S): Premenopausal women in the proliferative phase of the cycle.
INTERVENTION(S): The HES cells and primary human endometrial cells were exposed to cigarette smoke-saturated solution.
MAIN OUTCOME MEASURE(S): Cell proliferation and expression of different isoforms of nitric oxide synthase.
RESULT(S): Cigarette smoke inhibited HES cell proliferation in a dose- and time-dependant manner. The inhibitory effect of CS was blocked by hemoglobin and enhanced by L-arginine (L-Arg). Cigarette smoking and nicotine stimulated the expression of endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) whereas benzo[a]pyrene (BP) only stimulated the expression of eNOS in HES cells. Cigarette smoke stimulated the expression of eNOS/iNOS in primary human endometrial cells, comprised of epithelial and stromal cells. The effect of CS on eNOS/iNOS expression in HES cells was blocked by ascorbic acid but not by glutathione.
CONCLUSION(S): Cigarette smoke inhibits endometrial cell proliferation through a nitric oxide-mediated pathway.
确定香烟烟雾（CS）对人子宫内膜表面上皮细胞系增殖的直接影响。
使用 HES 细胞和原代人子宫内膜细胞的体外研究。
大学学术中心。
处于周期增殖期的绝经前妇女。
将 HES 细胞和原代人子宫内膜细胞暴露于香烟烟雾饱和溶液中。
细胞增殖和不同亚型一氧化氮合酶的表达。
香烟烟雾以剂量和时间依赖的方式抑制 HES 细胞增殖。CS 的抑制作用可被血红蛋白阻断，而被 L-精氨酸（L-Arg）增强。香烟烟雾和尼古丁刺激内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）的表达，而苯并[a]芘（BP）仅刺激 HES 细胞中 eNOS 的表达。香烟烟雾刺激原代人子宫内膜细胞（包括上皮细胞和基质细胞）中 eNOS/iNOS 的表达。CS 对 HES 细胞中 eNOS/iNOS 表达的影响可被抗坏血酸阻断，但不能被谷胱甘肽阻断。
香烟烟雾通过一氧化氮介导的途径抑制子宫内膜细胞增殖。
