Gehring W G
Institute of Reproductive Medicine, Springe.
Andrologia. 1987 Sep-Oct;19(5):544-50. doi: 10.1111/j.1439-0272.1987.tb01896.x.
Semen samples from 18 fertile men and 18 infertile men were evaluated both fresh and after freezing and thawing using traditional semen parameter, the hypoosmotic swelling test (HOS-test) and the bovine cervical mucus penetration test (BMP-test). Major differences in the semen analysis between the fertile and infertile men were observed for motility, morphology (head and tail defects), the bovine mucus penetration test and the hypoosmotic swelling test. Cryopreservation resulted in a decrease regarding motility, the BMP-test and the HOS-test. Percentagewise the losses due to cryopreservation were higher in the infertile group than in the fertile group. The results of this study show that the bovine mucus penetration test as well as the HOS-test are valuable additions to the routine semen analysis of fresh and cryopreserved spermatozoa.
使用传统精液参数、低渗肿胀试验(HOS试验)和牛宫颈黏液穿透试验(BMP试验),对18名有生育能力男性和18名不育男性的精液样本进行了新鲜状态以及冷冻和解冻后的评估。在有生育能力和不育男性之间的精液分析中,观察到在活力、形态(头部和尾部缺陷)、牛黏液穿透试验和低渗肿胀试验方面存在主要差异。冷冻保存导致活力、BMP试验和HOS试验出现下降。按百分比计算,不育组因冷冻保存造成的损失高于有生育能力组。本研究结果表明,牛黏液穿透试验以及HOS试验是新鲜和冷冻精子常规精液分析的有价值补充。