Schultz-Cherry S, Dybing J K, Davis N L, Williamson C, Suarez D L, Johnston R, Perdue M L
SE Poultry Research Laboratory, ARS, USDA, 934 College Station Road, Athens, Georgia 30605, USA.
Virology. 2000 Dec 5;278(1):55-9. doi: 10.1006/viro.2000.0635.
Venezuelan equine encephalitis virus replicon particles (VRP) containing the gene expressing hemagglutinin (HA) from the human Hong Kong Influenza A isolate (A/HK/156/97) were evaluated as vaccines in chicken embryos and young chicks. Expressed HA was readily detected in bird-tissue staining with anti-H5 HA antibody and in chicken cells infected with the replicon preparations following immunoprecipitation with monoclonal antibody. Birds challenged with a dose of the lethal parent virus were protected to different extents depending on the age of the bird. In ovo and 1-day-old inoculated animals that received no boost with the VRP were partially protected; birds 2 weeks of age were completely protected with a single dose of VRP.
含有来自人类香港甲型流感分离株（A/HK/156/97）血凝素（HA）表达基因的委内瑞拉马脑炎病毒复制子颗粒（VRP）在鸡胚和幼雏中作为疫苗进行了评估。在用抗H5 HA抗体进行鸟类组织染色以及用单克隆抗体免疫沉淀后感染复制子制剂的鸡细胞中，很容易检测到表达的HA。用致死亲本病毒剂量攻击的鸟类根据其年龄受到不同程度的保护。在卵内和未用VRP加强免疫的1日龄接种动物中受到部分保护；2周龄的鸟类用单剂量VRP完全得到保护。
