Josephy P D
Guelph-Waterloo Centre for Graduate Work in Chemistry and Biochemistry, Department of Chemistry and Biochemistry, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
Mutat Res. 2000 Nov 20;455(1-2):71-80. doi: 10.1016/s0027-5107(00)00063-4.
The Escherichia coli lacZ reversion assay, based on the set of episomal lacZ alleles engineered by Miller et al., provides an attractive system for studies of mutagenesis and mutational specificity. Each strain in the lacZ set reverts by a specific base substitution or frameshift event. Revertants are selected by growth on lactose minimal medium. In this review, I describe the development of the assay and its subsequent modifications and improvements. Examples of its application are presented and detailed protocols for the implementation of the assay are given.
基于米勒等人构建的附加体质粒lacZ等位基因集的大肠杆菌lacZ回复突变试验，为诱变和突变特异性研究提供了一个有吸引力的系统。lacZ基因集中的每个菌株通过特定的碱基替换或移码事件发生回复突变。通过在乳糖基本培养基上生长来筛选回复突变体。在这篇综述中，我描述了该试验的发展历程及其后续的修改和改进。文中展示了其应用实例，并给出了该试验实施的详细方案。
