Russo A
DBSF-Department of Structural and Functional Biology, University of Insubria, Via J.H. Dunant 3, 21100, Varese, Italy.
Mutat Res. 2000 Nov 20;455(1-2):167-89. doi: 10.1016/s0027-5107(00)00115-9.
This chapter summarizes the most relevant methodologies available for evaluation of cytogenetic damage induced in vivo in mammalian germ cells. Protocols are provided for the following endpoints: numerical and structural chromosome aberrations in secondary oocytes or first-cleavage zygotes, reciprocal translocations in primary spermatocytes, chromosome counting in secondary spermatocytes, numerical and structural chromosome aberrations, and sister chromatid exchanges (SCE) in spermatogonia, micronuclei in early spermatids, aneuploidy in mature sperm. The significance of each methodology is discussed. The contribution of novel molecular cytogenetic approaches to the detection of chromosome damage in rodent germ cells is also considered.
本章总结了可用于评估哺乳动物生殖细胞体内诱导的细胞遗传损伤的最相关方法。针对以下终点提供了实验方案：次级卵母细胞或第一次卵裂合子中的染色体数目和结构畸变、初级精母细胞中的相互易位、次级精母细胞中的染色体计数、精原细胞中的染色体数目和结构畸变以及姐妹染色单体交换（SCE）、早期精子细胞中的微核、成熟精子中的非整倍体。讨论了每种方法的意义。还考虑了新型分子细胞遗传学方法对啮齿动物生殖细胞染色体损伤检测的贡献。
