Albanese R
ICI Pharmaceuticals Division, Macclesfield, Cheshire, UK.
Mutagenesis. 1987 Mar;2(2):79-85. doi: 10.1093/mutage/2.2.79.
The methods used for the detection of chemically induced chromosome damage in male germ cells are discussed. These tests have been divided into direct and indirect cytogenetic methods. The direct methods assess chromosome damage in the dosed animal but analysis is restricted to the dividing spermatogonia and spermatocytes. Using indirect methods, chromosome damage is assessed in the F1 progeny of the dosed male and analysis covers all germ cell stages. Both methods can provide evidence of germ cell exposure but the data obtained from the indirect tests are considered more relevant since a positive result clearly constitutes unequivocal evidence of transmitted damage. The analysis of one-cell embryos from matings involving dosed parents is considered to be the most useful indirect test system since both structural and numerical aberrations in male and female F1 offspring can be assessed. Although relevant to the assessment of mutagenic hazard, the technically demanding methods used in the germ cell techniques prevent their use for preliminary screening programmes.
本文讨论了用于检测化学物质诱导雄性生殖细胞染色体损伤的方法。这些检测方法分为直接和间接细胞遗传学方法。直接方法评估受试动物的染色体损伤，但分析仅限于正在分裂的精原细胞和精母细胞。使用间接方法时，在受试雄性的F1代子代中评估染色体损伤，分析涵盖所有生殖细胞阶段。两种方法都可以提供生殖细胞暴露的证据，但从间接检测获得的数据被认为更具相关性，因为阳性结果显然构成了明确的遗传损伤证据。对涉及受试亲本交配产生的单细胞胚胎进行分析被认为是最有用的间接检测系统，因为可以评估雄性和雌性F1代子代中的结构和数目畸变。尽管与诱变危害评估相关，但生殖细胞技术中使用的技术要求较高的方法使其无法用于初步筛选程序。
