Sakolsky D J, Ashby B
Department of Pharmacology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA.
Brain Res Mol Brain Res. 2000 Dec 8;84(1-2):158-61. doi: 10.1016/s0169-328x(00)00239-4.
There is evidence that D1 and D2 dopamine receptors are co-expressed on some neurons. As a potential model of co-expression we examined Ntera-2 cells using RT-PCR, and showed that they express D2 but not D1 receptors. D2 dopamine receptor expression was confirmed by quinpirole inhibition of forskolin-stimulated cAMP formation. Absence of D1 dopamine receptors was confirmed by the inability of dopamine or SKF 81297 to increase cAMP.
有证据表明，D1和D2多巴胺受体在某些神经元上共同表达。作为共表达的潜在模型，我们使用逆转录聚合酶链反应（RT-PCR）检测了Ntera-2细胞，结果显示它们表达D2受体而非D1受体。喹吡罗对福斯高林刺激的环磷酸腺苷（cAMP）生成的抑制作用证实了D2多巴胺受体的表达。多巴胺或SKF 81297无法增加cAMP，这证实了D1多巴胺受体的缺失。
