Sanada H, Yao L, Jose P A, Carey R M, Felder R A
Department of Pathology, University of Virginia Center for the Health Sciences, Charlottesville 22908, USA.
Clin Exp Hypertens. 1997 Jan-Feb;19(1-2):93-105. doi: 10.3109/10641969709080807.
D2-like receptors in the kidney have been suggested to be important in the regulation of renin release but the D2-like subtype(s) expressed in juxtaglomerular (JG) cells is not known. Therefore, we determined which of the D2-like family of dopamine receptors is located in primary cultures of rat juxtaglomerular (JG) cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) identified D3 and D4 but not D2Long mRNA in JG cells (n = 3). D3 receptor function was demonstrated by a concentration-dependent inhibition of forskolin-stimulated cAMP production by LY-171555 (a non-selective D2-like receptor agonist) and PD-128593 (a partially selective D3 agonist) (n = 3-7/group). The stimulatory action of LY-171555 and PD-128593 we blocked by the non-selective D2-like antagonist YM-09151. We conclude that D3 and D4 dopamine receptor subtypes are expressed in JG cells; the receptor subtype linked to the inhibition of cAMP in JG cells remains to be established.
肾中的 D2 样受体被认为在肾素释放调节中起重要作用，但肾小球旁（JG）细胞中表达的 D2 样亚型尚不清楚。因此，我们确定了多巴胺受体 D2 样家族中的哪一种位于大鼠肾小球旁（JG）细胞的原代培养物中。逆转录聚合酶链反应（RT-PCR）在 JG 细胞（n = 3）中鉴定出 D3 和 D4，但未鉴定出 D2Long mRNA。LY-171555（一种非选择性 D2 样受体激动剂）和 PD-128593（一种部分选择性 D3 激动剂）对福斯可林刺激的 cAMP 产生的浓度依赖性抑制证明了 D3 受体功能（n = 3-7/组）。LY-171555 和 PD-128593 的刺激作用被非选择性 D2 样拮抗剂 YM-09151 阻断。我们得出结论，D3 和 D4 多巴胺受体亚型在 JG 细胞中表达；与 JG 细胞中 cAMP 抑制相关的受体亚型仍有待确定。
