Senft A W, Maddison S E
Am J Trop Med Hyg. 1975 Jan;24(1):83-9. doi: 10.4269/ajtmh.1975.24.83.
A proteolytic enzyme which hydrolyses hemoglobin was obtained from the supernatant fraction of homogenized Schistosoma mansoni. This enzyme elicited histaminic skin reactions in various animals, including man, which were infected with S. mansoni. It failed to induce reactions in monkeys harboring S. haematobium, S. japonicum, or S. intercalatum. In a preliminary field trial in the Caribbean, the skin test proved to be somewhat less sensitive than the customarily used extract of adult worms in Coca's solution. However, the enzyme appeared to induce fewer false positive reactions and delayed responses than did the Coca's extract. A new diagnostic test for schistosomiasis probably could be developed by using specific parasite enzymes against which the host has become sensitized in the course of infection.
一种能水解血红蛋白的蛋白水解酶是从曼氏血吸虫匀浆的上清液部分获得的。这种酶在包括人类在内的感染曼氏血吸虫的各种动物中引发组胺性皮肤反应。它未能在感染埃及血吸虫、日本血吸虫或间插血吸虫的猴子中诱导反应。在加勒比地区的一项初步现场试验中，皮肤试验被证明比通常使用的成虫在古柯溶液中的提取物灵敏度略低。然而，与古柯提取物相比，这种酶似乎诱导的假阳性反应和延迟反应较少。利用宿主在感染过程中已致敏的特定寄生虫酶，可能开发出一种新的血吸虫病诊断试验。
