Zhu X, Humphrey P A
Division of Surgical Pathology, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Urology. 2000 Dec 20;56(6):1071-4. doi: 10.1016/s0090-4295(00)00795-0.
Scatter factor (hepatocyte growth factor) (SF/HGF) is a multifunctional polypeptide growth factor that has been implicated in tumor proliferation, angiogenesis, invasiveness, and metastasis. Little is known of the expression of SF/HGF in human prostatic carcinoma. The aims of this investigation were to quantitate the level of SF/HGF expression in benign versus malignant human prostatic tissues and to assess regulation of SF/HGF expression by human prostatic stromal myofibroblasts.
We determined the level of SF/HGF expression in 10 human prostatic tissue samples (5 benign, 5 carcinoma) by Western blot analysis. Five purified growth factors-basic fibroblast growth factor (bFGF), interleukin-1beta (IL-1beta), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and endothelial growth factor (EGF)-were tested for their capacity to induce SF/HGF expression by a human prostatic stromal myofibroblastic cell line, as assessed by enzyme-linked immunosorbent assay. Supernatant from the normal PrEC prostatic epithelial cell line and the DU 145 carcinoma cell line were assayed for SF/HGF-inducing activity.
SF/HGF exhibited a mean fourfold overexpression in carcinoma tissues compared with benign prostatic tissue. Significant stimulation of SF/HGF expression by prostatic stromal myofibroblasts was detected for IL-1beta (8.1-fold), PDGF (6.2-fold), bFGF (4.0-fold), VEGF (3. 7-fold), and EGF (2.9-fold). DU 145-conditioned media, but not the PrEC-conditioned media, contained SF/HGF-inducing activity, which was determined to include IL-1beta, bFGF, and PDGF by antibody-blocking experiments.
SF/HGF is overexpressed in human prostatic carcinoma tissues. Prostatic carcinoma cell stimulation of SF/HGF expression by adjacent benign myofibroblastic cells as a type of epithelial-stromal paracrine interaction could potentially influence prostatic carcinoma cell behaviors.
分散因子（肝细胞生长因子）（SF/HGF）是一种多功能多肽生长因子，与肿瘤增殖、血管生成、侵袭及转移有关。关于SF/HGF在人类前列腺癌中的表达情况，人们了解甚少。本研究的目的是定量分析SF/HGF在人类前列腺良性与恶性组织中的表达水平，并评估人类前列腺基质肌成纤维细胞对SF/HGF表达的调控作用。
我们通过蛋白质免疫印迹分析确定了10份人类前列腺组织样本（5份良性，5份癌组织）中SF/HGF的表达水平。通过酶联免疫吸附测定法，检测了5种纯化的生长因子——碱性成纤维细胞生长因子（bFGF）、白细胞介素-1β（IL-1β）、血小板衍生生长因子（PDGF）、血管内皮生长因子（VEGF）和内皮生长因子（EGF）——诱导人类前列腺基质肌成纤维细胞系表达SF/HGF的能力。检测了正常前列腺上皮细胞系PrEC和DU 145癌细胞系的上清液中诱导SF/HGF的活性。
与良性前列腺组织相比，SF/HGF在癌组织中平均过表达四倍。检测到前列腺基质肌成纤维细胞对SF/HGF表达有显著刺激作用的有IL-1β（8.1倍）、PDGF（6.2倍）、bFGF（4.0倍）、VEGF（3.7倍）和EGF（2.9倍）。DU 145条件培养基而非PrEC条件培养基含有诱导SF/HGF的活性，通过抗体阻断实验确定其活性成分包括IL-1β、bFGF和PDGF。
SF/HGF在人类前列腺癌组织中过表达。作为一种上皮-基质旁分泌相互作用，相邻良性肌成纤维细胞对前列腺癌细胞表达SF/HGF的刺激可能会影响前列腺癌细胞的行为。
