Cogliatti S B, Griesser H, Peng H, Du M Q, Isaacson P G, Zimmermann D R, Maibach R C, Schmid U
Department of Pathology, State Hospital, St. Gallen, Switzerland.
J Pathol. 2000 Dec;192(4):470-8. doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH733>3.0.CO;2-U.
Fifty-five cases of primary extranodal high-grade B-cell non-Hodgkin's lymphoma were investigated for bcl-2 and p53 protein expression as well as for t(14;18) translocations and p53 mutations. Phenotypic and genotypic profiles were compared between tumours of gastric (27 cases) and non-gastric (28 cases) origin. bcl-2 protein expression was significantly lower in gastric (11/27) than in non-gastric (28/28) lymphomas (p<0.0001), while nuclear p53 protein expression did not differ significantly between these two groups. In the stomach, there were no significant differences in either bcl-2 or p53 expression profiles between high-grade lymphomas with (n=14) and without (n=13) evidence of a low-grade component of MALT type. However, secondary high-grade lymphomas showed a significant down-regulation of bcl-2 protein (p<0.0001) and, conversely, an up-regulation of p53 protein (p<0.0001) as compared with their low-grade tumour components. In extranodal high-grade B-cell lymphomas, bcl-2 protein expression was not associated with t(14;18) translocation. Only one gastric lymphoma had a p53 point mutation with potential alteration of the amino acid sequence. These findings indicate that primary gastric high-grade B-cell lymphomas are immunohistologically distinct from primary extranodal high-grade B-cell lymphomas of an origin other than in the stomach.
对55例原发性结外高级别B细胞非霍奇金淋巴瘤进行了bcl-2和p53蛋白表达检测，以及t(14;18)易位和p53突变检测。比较了胃源性（27例）和非胃源性（28例）肿瘤的表型和基因型特征。胃淋巴瘤（11/27）中bcl-2蛋白表达显著低于非胃淋巴瘤（28/28）（p<0.0001），而这两组之间核p53蛋白表达无显著差异。在胃中，有低度MALT型成分证据的高级别淋巴瘤（n=14）和无此证据的高级别淋巴瘤（n=13）之间，bcl-2或p53表达谱均无显著差异。然而，继发性高级别淋巴瘤与低度肿瘤成分相比，bcl-2蛋白显著下调（p<0.0001），相反，p53蛋白上调（p<0.0001）。在结外高级别B细胞淋巴瘤中，bcl-2蛋白表达与t(14;18)易位无关。只有1例胃淋巴瘤存在p53点突变，可能导致氨基酸序列改变。这些发现表明，原发性胃高级别B细胞淋巴瘤在免疫组织学上与胃外原发性结外高级别B细胞淋巴瘤不同。
