Ilyas M, Kendall M, Jalal H, Linton C, Rooney N
Cancer Genetics and Immunology Laboratory, John Radcliffe Hospital, Headington, Oxford, U.K.
J Pathol. 1996 Nov;180(3):249-53. doi: 10.1002/(SICI)1096-9896(199611)180:3<249::AID-PATH672>3.0.CO;2-8.
The aim of this study was to investigate the changes involved in the evolution of nine cases of recurrent B-cell lymphomas. Using the polymerase chain reaction (PCR) on formalin-fixed, paraffin-embedded tissue from both the primary and the recurrent lymphoma of each case, monoclonality was demonstrated in every tumour. In all nine cases, the recurrent lymphoma was shown to belong to the same clone as the primary lymphoma. Eight of these cases were then investigated by immunohistochemistry for changes in Bcl-2 and p53 expression. Five out of eight of the primary lymphomas showed Bcl-2 overexpression. Two of the three cases initially negative for Bcl-2 expression became positive in the recurrence. One out of eight of the primary lymphomas was positive for p53 expression. Of the seven negative cases, one became positive for p53 expression in the recurrence. Both of the p53-positive cases showed high-grade histology. This study shows that Bcl-2 overexpression is probably an important early event in the development of B-cell lymphomas, although it may occur as a post-neoplastic event. p53 mutation is probably more important as a late event and may be associated with high-grade transformation.
本研究旨在调查9例复发性B细胞淋巴瘤演变过程中的变化。对每例原发性和复发性淋巴瘤的福尔马林固定、石蜡包埋组织进行聚合酶链反应（PCR），结果显示每个肿瘤均呈单克隆性。在所有9例病例中，复发性淋巴瘤与原发性淋巴瘤属于同一克隆。随后，对其中8例病例进行免疫组织化学检查，以观察Bcl-2和p53表达的变化。8例原发性淋巴瘤中有5例显示Bcl-2过表达。最初3例Bcl-2表达阴性的病例中有2例在复发时转为阳性。8例原发性淋巴瘤中有1例p53表达阳性。在7例阴性病例中，有1例在复发时p53表达转为阳性。2例p53阳性病例均显示为高级别组织学类型。本研究表明，Bcl-2过表达可能是B细胞淋巴瘤发生过程中的一个重要早期事件，尽管它可能作为肿瘤后事件出现。p53突变可能作为晚期事件更为重要，并且可能与高级别转化有关。
