areCBA是不动杆菌属菌株ADP1中的一个操纵子，受AreR（一种依赖σ⁵⁴的调节因子）控制。
areCBA is an operon in Acinetobacter sp. strain ADP1 and Is controlled by AreR, a sigma(54)-dependent regulator.
作者信息
Jones R M, Williams P A
机构信息
School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom.
出版信息
J Bacteriol. 2001 Jan;183(1):405-9. doi: 10.1128/JB.183.1.405-409.2001.
Abstract
The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR, which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC, near a sigma(54)-dependent -12, -24 promoter. Inactivation of areR and rpoN (for RNA polymerase sigma(54)) drastically reduced growth rates on the Are substrates and induction of the operon.
摘要
不动杆菌属ADP1菌株中决定在苄基链烷酸酯上生长的areCBA基因，被证明作为一个单一操纵子进行转录，并受areR调控，areR编码一种NtrC/XylR家族的调控蛋白。对Are酶以及作为报告基因的两个lacZ插入片段的分析表明，该操纵子可被乙酸苄酯、苯甲醇、苯甲醛以及2-和4-羟基苄基乙酸酯、苄基丙酸酯和丁酸酯诱导。两个相邻的转录起始位点位于areC起始密码子上游97和96 bp处，靠近一个依赖于sigma(54)的-12、-24启动子。areR和rpoN（用于RNA聚合酶sigma(54)）的失活显著降低了在Are底物上的生长速率以及该操纵子的诱导。
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