Fraser P D, Pinto M E, Holloway D E, Bramley P M
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK.
Plant J. 2000 Nov;24(4):551-8. doi: 10.1046/j.1365-313x.2000.00896.x.
The application of high-performance liquid chromatography (HPLC) using a C30 reverse-phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana, and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.
使用C30反相固定基质的高效液相色谱法（HPLC）能够在一张色谱图中同时分离胡萝卜素、叶黄素、泛醌、生育酚和质体醌。连续光电二极管阵列（PDA）检测确保了洗脱时化合物的鉴定和定量。描述了该方法在具有改变的类异戊二烯含量的转基因和突变番茄品种表征、拟南芥生化筛选以及漂白除草剂作用模式阐明中的应用，以说明该程序的多功能性。
