Mimori K, Inoue H, Alder H, Ueo H, Tanaka Y, Mori M
Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
Oncol Rep. 2001 Jan-Feb;8(1):39-42.
hBUB1 is a human homolog of yeast mitotic check point gene that plays an important role in chromosome segregation. Recently mutations of hBUB1 were reported in colorectal cancer cell lines, indicating that inactivation of this gene could be directly involved in aneuploidy in human carcinoma cells. To obtain information of the magnitude of hBUB1 inactivation in multiple carcinomas, we examined mutations in 59 multiple carcinoma cell lines showing single base alteration, however, there was no mutation of hBUB1 with amino acid change in these carcinomas. There were four silent mutations at codon 93, codon 735, codon 430 and codon 98 in KYSE190, TE8 esophageal carcinoma cells, KATOIII gastric carcinoma cells and 697 B cell leukemia cells, respectively. Two candidates of mutation were identified in TE3 esophageal carcinoma cells and 697 B cell leukemia cell line at codon 9 and codon 285, respectively. This result suggests that the inactivation of hBUB1 may be very rare in human carcinomas, or restricted to certain cell lines of colorectal carcinomas.
hBUB1是酵母有丝分裂检查点基因的人类同源物，在染色体分离中起重要作用。最近在结肠癌细胞系中报道了hBUB1的突变，这表明该基因的失活可能直接参与人类癌细胞的非整倍体形成。为了获取多种癌症中hBUB1失活程度的信息，我们检测了59种显示单碱基改变的多种癌细胞系中的突变情况，然而，在这些癌症中没有发现hBUB1发生氨基酸改变的突变。在KYSE190细胞、TE8食管癌细胞、KATOIII胃癌细胞和697 B细胞白血病细胞中，分别在密码子93、密码子735、密码子430和密码子98处有四个沉默突变。在TE3食管癌细胞和697 B细胞白血病细胞系中分别在密码子9和密码子285处鉴定出两个突变候选位点。该结果表明，hBUB1的失活在人类癌症中可能非常罕见，或者仅限于某些结肠癌细胞系。
