Kakeji Y, Maehara Y, Koga T, Shibahara K, Kabashima A, Tokunaga E, Sugimachi K
Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Oncol Rep. 2001 Jan-Feb;8(1):107-10.
Telomerase activity was reported to be activated in most immortal cells and cancers. As the clinical significance of telomerase activity in human gastric cancer is controversial, we investigated this activity using a telomeric repeat amplification protocol. The telomerase activity was tentatively defined by strength of activity as follows: 3+, observed with 0.06 microg of protein; 2+, observed with 0.6 microg of protein; 1+, observed with 6 microg of protein; 0, not observed under these three conditions. Telomerase activity was detected in 35 of 39 (89.7%) gastric cancer specimens. Tumors with high telomerase activities (2+/3+) tended to have a deeper invasion, lymphatic and vascular invasion, lymph node metastasis, liver metastasis, and peritoneal dissemination, as compared to findings in case of low telomerase activities (-/1+). Thus, telomerase activity of gastric cancer tissue may reflect the malignant potential of the tumor and intensive postoperative care might be required for such patients.
据报道，端粒酶活性在大多数永生化细胞和癌症中被激活。由于端粒酶活性在人类胃癌中的临床意义存在争议，我们使用端粒重复序列扩增法对其活性进行了研究。端粒酶活性根据活性强度初步定义如下：3+，在0.06微克蛋白质时观察到；2+，在0.6微克蛋白质时观察到；1+，在6微克蛋白质时观察到；0，在这三种条件下均未观察到。在39例胃癌标本中的35例（89.7%）检测到端粒酶活性。与端粒酶活性低（-/1+）的情况相比，端粒酶活性高（2+/3+）的肿瘤往往具有更深的浸润、淋巴管和血管浸润、淋巴结转移、肝转移和腹膜播散。因此，胃癌组织的端粒酶活性可能反映肿瘤的恶性潜能，此类患者术后可能需要加强护理。
