Ma Z J, Yamaguchi M
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Int J Mol Med. 2001 Jan;7(1):73-8. doi: 10.3892/ijmm.7.1.73.
The effect of zinc and growth factor on bone protein component in newborn rats was investigated. The characterization of protein component in the femoral-diaphyseal and metaphyseal tissue of newborn rats (3-35 days old) was examined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The diaphyseal and metaphyseal tissues of 7 days-old rats were cultured for 24 or 48 h in a medium containing either vehicle, zinc sulfate (10-4 M) or dipicolinate (10-3 M), a chelator of zinc ion, in the presence or absence of insulin-like growth factor-I (IGF-I; 10-8 M) or transforming growth factor-beta (TFG-beta; 10-10 M) with an effective concentration. Many cellular protein molecules were present in the diaphyseal and metaphyseal tissues; potent bands were seen in protein molecules with about 66 and 46 kDa. Protein molecule of about 66 kDa was greatly released in the medium cultured with the diaphyseal and metaphyseal tissues. This protein in the medium was increased by culture with zinc, IGF-I or TGF-beta. Total protein content in the medium cultured with the diaphyseal and metaphyseal tissues was significantly increased in the presence of zinc, IGF-I or TGF-beta. The IGF-I-increased medium protein content was significantly enhanced by zinc. This enhancing effect was not seen in TGF-beta. Alkaline phosphatase activity and deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues was significantly increased by culture with zinc, IGF-I or TGF-beta. The effect of IGF-I was significantly enhanced by zinc, while it was not found in TGF-beta. The effect of IGF-I or TGF-beta in increasing the bone components was seen in the presence of dipicolinate. This study demonstrates that zinc, like IGF-I and TGF-beta, can increase protein components in the femoral-diaphyseal and metaphyseal tissues of new-born rats. Zinc may especially play a role in bone growth in collaboration with IGF-I.
研究了锌和生长因子对新生大鼠骨蛋白成分的影响。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，检测新生大鼠（3 - 35日龄）股骨干和干骺端组织中蛋白成分的特征。将7日龄大鼠的干骺端和干骺端组织在含有载体、硫酸锌（10⁻⁴M）或吡啶二羧酸（10⁻³M，锌离子螯合剂）的培养基中培养24或48小时，同时存在或不存在有效浓度的胰岛素样生长因子-I（IGF-I；10⁻⁸M）或转化生长因子-β（TGF-β；10⁻¹⁰M）。在干骺端和干骺端组织中存在许多细胞蛋白分子；在约66和46 kDa的蛋白分子中可见强条带。约66 kDa的蛋白分子在干骺端和干骺端组织培养的培养基中大量释放。培养基中的这种蛋白通过锌、IGF-I或TGF-β培养而增加。在锌、IGF-I或TGF-β存在下，干骺端和干骺端组织培养的培养基中的总蛋白含量显著增加。锌显著增强了IGF-I增加培养基蛋白含量的作用。TGF-β未观察到这种增强作用。锌、IGF-I或TGF-β培养显著增加了干骺端和干骺端组织中的碱性磷酸酶活性和脱氧核糖核酸（DNA）含量。锌显著增强了IGF-I的作用，而TGF-β未观察到这种作用。在吡啶二羧酸存在下可见IGF-I或TGF-β增加骨成分的作用。本研究表明，锌与IGF-I和TGF-β一样，可增加新生大鼠股骨干和干骺端组织中的蛋白成分。锌可能尤其在与IGF-I协同促进骨骼生长中发挥作用。
