Petukhov V N, Fomchenkov V M, Chugunov V A, Kholodenko V P
State Scientific Center of Applied Microbiology, Obolensk, Moscow oblast, 142279 Russia.
Prikl Biokhim Mikrobiol. 2000 Nov-Dec;36(6):652-5.
Reactiona of higher plants (mustard, oat, rye, salad, dill and barley) and microalgae (Euglena gracilis) on the contamination of soil and water with petroleum and oil products was studied. The germination of seeds was analyzed. The length of sprouts, dry biomass and length of plant roots, as well as the optical density of micro-algal broth culture were determined. Negative effects of soil and water contamination with petroleum and oil products on plant and microalgal parameters examined was shown. After biological destruction of contaminants by an association of destructor strains (Acinetobacter sp., Mycobacterium flavescens and Rhodoccocus sp.), the toxicity of contaminated mediums decreased. The data suggest that the integral toxicity of soil and water contaminated with petroleum and oil products and toxicity change during biodestruction of these pollutants can be analyzed by using plant test organisms.
研究了高等植物（芥菜、燕麦、黑麦、生菜、莳萝和大麦）和微藻（纤细裸藻）对土壤和水体中石油及石油产品污染的反应。分析了种子的发芽情况。测定了芽的长度、干生物量、植物根的长度以及微藻肉汤培养物的光密度。结果表明，土壤和水体中石油及石油产品污染对所检测的植物和微藻参数具有负面影响。在由降解菌株联合体（不动杆菌属、微黄分枝杆菌和红球菌属）对污染物进行生物降解后，受污染介质的毒性降低。数据表明，利用植物测试生物可以分析被石油及石油产品污染的土壤和水体的综合毒性以及这些污染物生物降解过程中的毒性变化。
