Stewart I J
Dept of Biomedical Science, Institute of Medical Sciences, University of Aberdeen, Foresterhill, UK.
J Anat. 2000 Oct;197 Pt 3(Pt 3):495-502. doi: 10.1046/j.1469-7580.2000.19730495.x.
Granulated metrial gland (GMG) cells differentiate in the uterine wall in pregnancy in mice but the mechanisms which control their differentiation and maintenance are unknown. In vivo, GMG cells share an intimate association with fibroblast-like stromal cells. The importance of this association has been assessed by examining the effects of withdrawal of stromal cell contact on GMG cell maintenance in vitro. When single cell suspensions of cells were prepared from mouse metrial glands there was a steady decline in numbers with days of culture but usually some remained at 7 d of culture. The ability of metrial gland cells to kill Wehi 164 target cells in 51Cr-release cytotoxicity assays was retained by cells cultured for at least 3 d. When explants of metrial gland were maintained in culture to allow GMG cell migration onto the culture flask, the attached GMG cells were lost by 1 d later. Overall, these results suggest that a juxtacrine regulatory mechanism maintains GMG cells. The rapid loss of unsupported GMG cells in culture has major implications in the design of assays to examine GMG cell function.
颗粒状蜕膜腺（GMG）细胞在小鼠孕期子宫壁中分化，但其分化和维持的机制尚不清楚。在体内，GMG细胞与成纤维细胞样基质细胞密切相关。通过检测体外去除基质细胞接触对GMG细胞维持的影响，评估了这种关联的重要性。从小鼠蜕膜腺制备细胞单细胞悬液时，细胞数量随培养天数稳步下降，但通常在培养7天时仍有一些细胞留存。在51Cr释放细胞毒性试验中，培养至少3天的蜕膜腺细胞保留了杀伤Wehi 164靶细胞的能力。当蜕膜腺外植体在培养中维持以允许GMG细胞迁移到培养瓶上时，附着的GMG细胞在1天后丢失。总体而言，这些结果表明旁分泌调节机制维持着GMG细胞。培养中无支持的GMG细胞的快速丢失对检测GMG细胞功能的试验设计具有重要意义。
