DLC-1在原发性肝细胞癌中缺失，并对DLC-1缺失的肝癌细胞系增殖发挥抑制作用。

DLC-1 is deleted in primary hepatocellular carcinoma and exerts inhibitory effects on the proliferation of hepatoma cell lines with deleted DLC-1.

作者信息

Ng I O, Liang Z D, Cao L, Lee T K

机构信息

Department of Pathology, University of Hong Kong, Queen Mary Hospital, China.

出版信息

Cancer Res. 2000 Dec 1;60(23):6581-4.

PMID:11118037

Abstract

We investigated the expression and deletion of DLC-1 (frequently deleted in liver cancer gene), first reported in 1998 and having a high homology with rat p122RhoGAP in hepatocellular carcinoma (HCC). Six (20%) of 30 human HCC samples and 2 (40%) of 5 HCC cell lines were found to have no detectable DLC-1 expression by reverse transcription-PCR. Homozygous DLC-1 deletion was detected by Southern blotting in two of six HCC samples and in both HCC cell lines with no DLC-1 expression. Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. Our findings suggest that DLC-1 may play an important role in hepatocarcinogenesis.

摘要

我们研究了肝癌缺失基因1（DLC-1，1998年首次报道，与大鼠p122RhoGAP在肝细胞癌（HCC）中具有高度同源性）的表达和缺失情况。通过逆转录聚合酶链反应（RT-PCR）发现，30例人类肝癌样本中有6例（20%）以及5种肝癌细胞系中有2例（40%）未检测到DLC-1表达。通过Southern印迹法在6例肝癌样本中的2例以及2种无DLC-1表达的肝癌细胞系中检测到了DLC-1纯合缺失。将DLC-1转染到5种肝癌细胞系（2种有DLC-1缺失，3种有完整的DLC-1）中，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和集落形成试验发现，在这2种有DLC-1缺失的肝癌细胞系中出现了显著的生长抑制，而在另外3种有完整DLC-1的肝癌细胞系中则未出现。我们的研究结果表明，DLC-1可能在肝癌发生过程中起重要作用。