Gea-Banacloche J C, Martino L, Mican J M, Hallahan C W, Baseler M, Stevens R, Lambert L, Polis M, Lane H C, Connors M
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
AIDS Res Hum Retroviruses. 2000 Nov 20;16(17):1877-86. doi: 10.1089/08892220050195838.
Although skewing of the CD4+ TCR repertoire in advanced HIV infection is well documented, increases in polyclonality during antiretroviral therapy have been less consistently observed. Ten patients, each with documented abnormalities within the CD4+ TCR repertoire, were studied by CDR3 spectratyping, semiquantitative PCR, and SSCP during 9-26 months of therapy. Naive and memory cell phenotypes were analyzed by flow cytometry. Six of 10 patients showed increased polyclonality of their TCR repertoires, 1 showed no change, and 3 showed increased TCR skewing, despite suppressed viral replication. Overall, there was no significant change in the percentage of abnormal BV subfamilies (from a mean of 25.5 to 17.1%) or the percentage of naive CD4+ T cells (from a mean of 18 to 25%). Further, progression of TCR repertoire disruptions was observed in some patients even with suppression of plasma viral RNA below 500 copies/ml. Although a spectrum of changes may be seen within the CD4+ TCR repertoire in the setting of antiretroviral therapy, increases in polyclonality are observed in some patients.
尽管在晚期HIV感染中CD4+T细胞受体库的偏斜已有充分记录，但在抗逆转录病毒治疗期间多克隆性增加的现象却较少得到一致观察。通过CDR3谱型分析、半定量PCR和单链构象多态性分析，对10例CD4+T细胞受体库有记录异常的患者在治疗9至26个月期间进行了研究。通过流式细胞术分析初始和记忆细胞表型。10例患者中有6例显示其T细胞受体库的多克隆性增加，1例无变化，3例显示T细胞受体偏斜增加，尽管病毒复制受到抑制。总体而言，异常BV亚家族的百分比（从平均25.5%降至17.1%）或初始CD4+T细胞的百分比（从平均18%升至25%）没有显著变化。此外，即使血浆病毒RNA被抑制到低于500拷贝/毫升，仍有一些患者观察到T细胞受体库破坏的进展。尽管在抗逆转录病毒治疗背景下CD4+T细胞受体库可能会出现一系列变化，但一些患者中仍观察到多克隆性增加。
