Pettersson A, Boketoft A, Sabirsh A, Nilsson N E, Kotarsky K, Olde B, Owman C
Division of Molecular Neurobiology, Wallenberg Neuroscience Center, Lund, Sweden.
Biochem Biophys Res Commun. 2000 Dec 20;279(2):520-5. doi: 10.1006/bbrc.2000.3989.
The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.
白三烯B4受体（BLTR）是一种七跨膜趋化因子受体，在促炎反应中起重要作用。我们制备了首个广泛适用的抗人BLTR单克隆抗体，并通过对稳定表达重组受体的三种不同细胞系进行流式细胞术分析，证实了抗体的特异性。这些抗体不与最近克隆的第二个白三烯B4受体BLTR2、半胱氨酰白三烯1型（Cys LT1）受体或半胱氨酰白三烯2型（Cys LT2）受体发生交叉反应。结合双色流式细胞术进行的功能分析表明，BLTR抗体可与被白三烯B4激活的细胞结合。这些抗体在细胞酶联免疫吸附测定（ELISA）和免疫细胞化学中显示能识别BLTR。利用这些抗体还证实了BLTR在CD15阳性血液白细胞和分化的HL-60细胞中的内源性表达。
