Porta H, Rocha-Sosa M
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional de Autonoma Mexico, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
Biochim Biophys Acta. 2000 Dec 15;1517(1):139-42. doi: 10.1016/s0167-4781(00)00230-x.
A genomic clone encoding a common bean lipoxygenase (PvLOX5) was isolated from a Phaseolus vulgaris library. Reverse transcription-polymerase chain reaction analysis revealed that PvLOX5 is expressed during nodule development and in Rhizobium tropici inoculated roots. There was no detectable expression of PvLOX5 in non-inoculated roots, healthy leaves, leaves after Pseudomonas syringae pv. tabaci infection, floral buds or dry seeds.
从菜豆文库中分离出一个编码菜豆脂氧合酶（PvLOX5）的基因组克隆。逆转录-聚合酶链反应分析表明，PvLOX5在根瘤发育过程中以及在接种了热带根瘤菌的根中表达。在未接种的根、健康叶片、接种丁香假单胞菌烟草致病变种后的叶片、花芽或干种子中未检测到PvLOX5的表达。
