Muskens J, Barkema H W, Russchen E, van Maanen K, Schukken Y H, Bakker D
Animal Health Service, P.O. Box 4, 5280 AA, Boxtel, The Netherlands.
Vet Microbiol. 2000 Dec 20;77(3-4):253-61. doi: 10.1016/s0378-1135(00)00310-2.
In the Netherlands a survey was conducted to estimate the prevalence of paratuberculosis in dairy herds. In total 15822 cows of at least 3 years of age, belonging to 378 herds were tested using an absorbed ELISA. Of these herds, 55% (n=207) had one or more serologically positive cows. Of the positive non-vaccinated herds, most had one (n=98) or two (n=49) positive cows. The percentage positive cows per herd was 2.5+/-3.2%.The true prevalences on cow and herd levels, based on a test sensitivity that ranged from 0.3 to 0.4 and a specificity that ranged from 0.985 and 0.995, were estimated at 2. 7-6.9% and 31-71%. Seven herds had been vaccinated against paratuberculosis and these herds had a significantly higher percentage of serologically positive cows (23%) than the non-vaccinated herds (2.5%). In conclusion, a small percentage of the dairy cows and a high percentage of the dairy herds in the Netherlands is serologically positive. The percentages true infected cows and herds are difficult to estimate precisely due to uncertainties in test sensitivity and specificity.
在荷兰开展了一项调查，以估计奶牛群中副结核病的患病率。使用吸附酶联免疫吸附测定法（ELISA）对隶属于378个牛群的总共15822头至少3岁的奶牛进行了检测。在这些牛群中，55%（n = 207）有一头或多头血清学检测呈阳性的奶牛。在阳性的未接种疫苗的牛群中，大多数有一头（n = 98）或两头（n = 49）阳性奶牛。每个牛群中阳性奶牛的百分比为2.5±3.2%。基于0.3至0.4的检测灵敏度和0.985至0.995的特异性，估计奶牛和牛群水平上的真实患病率分别为2.7 - 6.9%和31 - 71%。有七个牛群接种了副结核病疫苗，这些牛群血清学检测呈阳性的奶牛百分比（23%）显著高于未接种疫苗的牛群（2.5%）。总之，荷兰一小部分奶牛和一大部分奶牛群血清学检测呈阳性。由于检测灵敏度和特异性存在不确定性，难以精确估计真正感染奶牛和牛群的百分比。
