Corpa J M, Pérez V, García Marín J F
Departamento de Patología Animal: Medicina Animal (Anatomía Patológica), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, s/n, 24071, León, Spain.
Vet Microbiol. 2000 Dec 20;77(3-4):475-85. doi: 10.1016/s0378-1135(00)00332-1.
In order to evaluate and compare the peripheral immune responses induced by the vaccination against paratuberculosis in relation with the age of immunization, two groups of lambs and goat kids were vaccinated at 15 days and 5 months old, respectively. A heat-killed commercial vaccine was inoculated subcutaneously and humoral and cellular immune responses were measured by an ELISA and IFN-gamma assay, respectively, at 0, 30, 90, 180, 270 and 360 dpv in the lambs and 0, 30, 90 and 180 dpv in the caprine. IFN-gamma values did not show statistically significant differences between both groups, but when compared to the unvaccinated controls, this cytokine response tend to disappear earlier in animals vaccinated at 15 days old. The antibody response was always higher and more persistent in animals vaccinated at 5 months. The possibility of the incomplete degree of maturation of the immune system in 15 days old animals as the cause of the differences in the immune response to vaccination is suggested.
为了评估和比较与免疫接种年龄相关的副结核病疫苗接种所诱导的外周免疫反应，分别对两组羔羊和山羊羔在15日龄和5月龄时进行了疫苗接种。皮下接种一种热灭活商业疫苗，并分别在羔羊接种后0、30、90、180、270和360天以及山羊接种后0、30、90和180天，通过ELISA和IFN-γ检测法测量体液免疫和细胞免疫反应。两组之间的IFN-γ值没有显示出统计学上的显著差异，但与未接种疫苗的对照组相比，15日龄接种疫苗的动物中这种细胞因子反应往往消失得更早。5月龄接种疫苗的动物抗体反应始终更高且更持久。有人提出，15日龄动物免疫系统成熟程度不完全可能是疫苗接种免疫反应存在差异的原因。
