Schnabel C A, Selleri L, Jacobs Y, Warnke R, Cleary M L
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA.
Mech Dev. 2001 Jan;100(1):131-5. doi: 10.1016/s0925-4773(00)00516-5.
Mammalian Pbx genes (Pbx1-3) encode a family of TALE homeodomain proteins that function as transcriptional regulators in numerous cell types (Curr. Opin. Genet. Dev. 8 (1998) 423). The present study highlights distinctive features of Pbx1b expression during mouse embryonic development as a framework to understand its biological functions. Immunohistochemical analyses demonstrate extensive expression of Pbx1b throughout post-implantation development, with highest levels observed during early to mid-gestation. Its initial distribution is predominantly associated with condensing mesoderm, however, Pbx1b displays dynamic expression patterns in derivatives of all principal germ layers. In particular, Pbx1b localizes to sites of mesenchymal-epithelial interactions during periods of active morphogenesis in tissues such as the lung, kidney, tooth buds and vibrissae follicles. Furthermore, BrdU labeling studies reveal that Pbx1b expression domains partially overlap with regions of cellular proliferation. Taken together, these data suggest that Pbx1b contributes to multiple cellular processes during embryogenesis, which may include roles in cell-autonomous regulation as well as in the mediation of tissue interactions.
哺乳动物的Pbx基因（Pbx1 - 3）编码一类TALE同源结构域蛋白家族，这些蛋白在多种细胞类型中作为转录调节因子发挥作用（《当代遗传学与发育生物学观点》8 (1998) 423）。本研究着重阐述了小鼠胚胎发育过程中Pbx1b表达的独特特征，以此作为理解其生物学功能的框架。免疫组织化学分析表明，Pbx1b在植入后发育过程中广泛表达，在妊娠早期至中期表达水平最高。其最初的分布主要与正在凝聚的中胚层相关，然而，Pbx1b在所有主要胚层的衍生物中呈现动态表达模式。特别是，在肺、肾、牙胚和触须毛囊等组织的活跃形态发生期，Pbx1b定位于间充质 - 上皮相互作用的部位。此外，BrdU标记研究表明，Pbx1b表达域与细胞增殖区域部分重叠。综上所述，这些数据表明Pbx1b在胚胎发生过程中参与多种细胞过程，这可能包括在细胞自主调节以及组织相互作用介导中的作用。
