鲤（Cyprinus carpio）和虹鳟（Oncorhynchus mykiss）血浆低密度脂蛋白和高密度脂蛋白中缩醛磷脂的检测
Detection of plasmalogen from plasma low density lipoprotein and high density lipoprotein in carp, Cyprinus carpio, and rainbow trout, Oncorhynchus mykiss.
作者信息
Yamaguchi T, Miyamoto K, Yagi S, Horigane A, Sato M, Takeuchi M
机构信息
Laboratory of Marine Biochemistry, Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori-Amamiya, Aoba-ku, 981-8555, Sendai, Japan.
出版信息
Comp Biochem Physiol A Mol Integr Physiol. 2000 Nov;127(3):339-46. doi: 10.1016/s1095-6433(00)00262-2.
The study revealed the presence of plasmalogens in the low density lipoprotein (LDL) and high density lipoprotein (HDL) of the fish. The composition of the plasmalogen in the carp plasma LDL phospholipids was 0.94 and 0.23% in the HDL; the LDL phospholipids in the rainbow trout were 0.44% and the HDL was 0.18%. Aldehydes from the plasmalogen were derivatized with dansylhydrazides and separated by high performance liquid chromatography (HPLC). Their presence was detected using a fluorescence detector. Hexadecanal (C16: 0), octadecanal (C18: 0) and octadecenal (C18: 1) were determined to be the major components in the carp and rainbow trout.
该研究揭示了鱼类低密度脂蛋白（LDL）和高密度脂蛋白（HDL）中存在缩醛磷脂。鲤鱼血浆LDL磷脂中缩醛磷脂的组成在HDL中为0.94%和0.23%；虹鳟鱼的LDL磷脂为0.44%，HDL为0.18%。缩醛磷脂中的醛用丹磺酰肼衍生化，并用高效液相色谱（HPLC）分离。使用荧光检测器检测它们的存在。已确定十六醛（C16:0）、十八醛（C18:0）和十八碳烯醛（C18:1）是鲤鱼和虹鳟鱼中的主要成分。
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