Muñana K R, Vitek S M, Hegarty B C, Kordick D L, Breitschwerdt E B
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.
Infect Immun. 2001 Jan;69(1):564-9. doi: 10.1128/IAI.69.1.564-569.2001.
Bartonella henselae is known to cause central nervous system (CNS) disease in humans, and neurological signs have been observed in experimentally infected cats. However, the pathogenesis of CNS disease remains unclear. This study was undertaken to determine whether B. henselae infects feline fetal brain cells in vitro. Microglial-cell- and astrocyte-enriched cultures were inoculated with B. henselae. Giménez staining identified bacterial organisms within microglial cells by day 7 postinoculation. The viability of the intracellular bacteria was demonstrated by incubating cultures with gentamicin and plating cell lysate on agar. Electron microscopy identified intracellular organisms with characteristic Bartonella morphology but identified no ultrastructural abnormalities within infected microglial cells. No evidence of infection was seen in Bartonella-inoculated astrocyte cultures. These findings suggest a role for microglia in the pathogenesis of B. henselae-associated neurological disease.
已知亨氏巴尔通体可导致人类中枢神经系统（CNS）疾病，并且在实验感染的猫中观察到了神经学症状。然而，CNS疾病的发病机制仍不清楚。本研究旨在确定亨氏巴尔通体在体外是否感染猫胎儿脑细胞。用亨氏巴尔通体接种富含小胶质细胞和星形胶质细胞的培养物。吉姆萨染色在接种后第7天在小胶质细胞内鉴定出细菌。通过用庆大霉素孵育培养物并将细胞裂解物接种在琼脂上，证明了细胞内细菌的活力。电子显微镜鉴定出具有特征性巴尔通体形态的细胞内生物体，但在感染的小胶质细胞内未发现超微结构异常。在接种巴尔通体的星形胶质细胞培养物中未发现感染迹象。这些发现表明小胶质细胞在亨氏巴尔通体相关神经疾病的发病机制中起作用。
