Sakata M, Kobayashi H, Sun G W, Mochizuki O, Takagi A, Kojima T
Department of Obstetrics and Gynecology, Seirei-Mikatabara General Hospital, Shizuoka, Japan.
Fertil Steril. 2000 Dec;74(6):1208-14. doi: 10.1016/s0015-0282(00)01587-9.
To evaluate the presence of ryudocan in follicular fluid (FF) and its possible correlation with FF E(2) and P, and to study the levels of ryudocan in granulosa-lutein cells stimulated with hCG.
Controlled clinical study and in vitro experiment.
University teaching hospital.
PATIENT(S): One hundred seven patients undergoing IVF.
INTERVENTION(S): The FF and granulosa-lutein cells were aspirated from follicles 34 hours after an ovulatory gonadotropin bolus.
MAIN OUTCOME MEASURE(S): FF ryudocan, E(2), and P levels as well as hCG-mediated induction of ryudocan.
RESULT(S): Ryudocan was abundant in the FF; the concentration of ryudocan in human FF was estimated to be 305.5 +/- 200.8 ng/mL (mean +/- SD). Atretic follicles had higher concentrations of ryudocan (559.1 +/- 156.5 ng/mL). FF ryudocan levels were inversely correlated with FF E(2) (r = -0.5023) and P concentrations (r = -0.4459). A detectable amount of ryudocan was found in pooled granulosa-lutein cells. Ryudocan production was augmented by surge levels of hCG.
CONCLUSION(S): Ryudocan is expressed in luteinized granulosa cells in vitro. The higher concentrations of ryudocan in FF of atretic follicles suggest an involvement of ryudocan in the process of atresia.
评估卵泡液（FF）中柳豆素的存在及其与FF中E（2）和P的可能相关性，并研究人绒毛膜促性腺激素（hCG）刺激后颗粒黄体细胞中柳豆素的水平。
对照临床研究和体外实验。
大学教学医院。
107例行体外受精（IVF）的患者。
在排卵促性腺激素推注后34小时从卵泡中抽吸FF和颗粒黄体细胞。
FF柳豆素、E（2）和P水平以及hCG介导的柳豆素诱导。
柳豆素在FF中含量丰富；人FF中柳豆素的浓度估计为305.5±200.8 ng/mL（均值±标准差）。闭锁卵泡中柳豆素浓度更高（559.1±156.5 ng/mL）。FF柳豆素水平与FF E（2）（r = -0.5023）和P浓度（r = -0.4459）呈负相关。在合并的颗粒黄体细胞中发现了可检测量的柳豆素。hCG的峰值水平可增加柳豆素的产生。
柳豆素在体外的黄体化颗粒细胞中表达。闭锁卵泡FF中柳豆素浓度较高，提示柳豆素参与闭锁过程。
