Goldenberg S, Vincent A, Scherrer K
Nucleic Acids Res. 1979 Jun 25;6(8):2787-97. doi: 10.1093/nar/6.8.2787.
Purified 15 S globin mRNA-protein (mRNP) complexes obtained by EDTA dissociation of duck reticulocytes polyribosomes were digested with the calcium dependant Staphylococcus aureus nuclease (EC 3. 1. 4. 7.). 25% of the globin mRNA sequences were resistant to extensive nuclease digestion as determined by TCA precipitation of the digested 15 S particles labelled in vivo with tritiated uridine. Polyacrylamide gel electrophoresis of the RNA from nuclease digested 15 S particles showed that the protected oligoribonucleotides were distributed into two distinct size classes of 25,000 and 12,000 MW. Comparison between in vitro iodine-labelled 9 S globin mRNA extracted from Staphylococcal nuclease digested 15 S mRNP particles was carried out by fingerprinting. Mapping of T1 ribonuclease digests by high-voltage electrophoresis and homochromatography showed that specific oligoribonucleotides were protected against nuclease attack by proteins of the 15 S mRNP.
通过用乙二胺四乙酸（EDTA）解离鸭网织红细胞多核糖体获得的纯化15S球蛋白信使核糖核酸 - 蛋白质（mRNP）复合物，用依赖钙的金黄色葡萄球菌核酸酶（EC 3.1.4.7）进行消化。如通过用氚化尿苷在体内标记的15S颗粒的消化产物的三氯乙酸沉淀所确定的，25%的球蛋白信使核糖核酸序列对广泛的核酸酶消化具有抗性。来自核酸酶消化的15S颗粒的核糖核酸的聚丙烯酰胺凝胶电泳表明，受保护的寡核糖核苷酸分布在两个不同的分子量类别中，分别为25,000和12,000道尔顿。通过指纹图谱对从金黄色葡萄球菌核酸酶消化的15S mRNP颗粒中提取的体外碘标记的9S球蛋白信使核糖核酸进行了比较。通过高压电泳和同系层析对T1核糖核酸酶消化产物进行图谱分析表明，特定的寡核糖核苷酸受到15S mRNP蛋白质的保护而免受核酸酶攻击。
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