Robertson H D, Dickson E, Model P, Prensky W
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3260-4. doi: 10.1073/pnas.70.11.3260.
Several techniques of RNA and DNA finger-printing and determination of sequence have been applied to nucleic acids labeled with (125)I. Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination method shows little sensitivity to potential structure in single-stranded RNA molecules, yields stable oligonucleotide products in a reproducible manner, and does not change the specificity of several ribonucleases and deoxyribonucleases.
几种RNA和DNA指纹分析及序列测定技术已应用于用（125）I标记的核酸。人5S RNA和噬菌体f2 RNA的指纹图谱在复杂性和特定模式上都与它们未碘化的对应物相似。因此，这里使用的碘化是一种通用的标记方法，主要似乎是标记胞嘧啶残基。这种碘化方法对单链RNA分子中的潜在结构敏感性较低，以可重复的方式产生稳定的寡核苷酸产物，并且不会改变几种核糖核酸酶和脱氧核糖核酸酶的特异性。
