Padovan C M, Del Bel E A, Guimarães F S
Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Ribeirão Preto, Brazil.
Pharmacol Biochem Behav. 2000 Oct;67(2):325-30. doi: 10.1016/s0091-3057(00)00361-0.
The objective of the study was to investigate the influence of restraint stress on the effects of 2-amino-7-phosphonoheptanoic acid (AP7), an NMDA receptor antagonist, injected into the hippocampus of rats submitted to the elevated plus maze (EPM). Male Wistar rats with cannulas aimed to the dorsal hippocampus were forced immobilized for 2 h. Twenty four hours later they received bilateral injections of saline or AP7 (10 nmol/0.5 microl), and were tested in the EPM. In another experiment the animals received the treatment immediately before or after the restraint period, and were tested in the EPM 24 h later. AP7 had no effect in any anxiety measure in non-stressed rats. In stressed animals the drug increased the percentage of open arm entries when injected before the test in the EPM. When administered immediately after the restraint period, AP7 increased the percentage of time spent in the open arms and tended to do the same with the percentage of entries in these same arms. The results suggest that interference with hippocampal NMDA receptors modify the anxiogenic effect of restraint stress in an EPM.
本研究的目的是调查束缚应激对注射到高架十字迷宫（EPM）实验大鼠海马体中的N-甲基-D-天冬氨酸（NMDA）受体拮抗剂2-氨基-7-磷酸庚酸（AP7）作用的影响。将带有插入背侧海马体套管的雄性Wistar大鼠强制固定2小时。24小时后，它们接受双侧注射生理盐水或AP7（10纳摩尔/0.5微升），并在EPM中进行测试。在另一项实验中，动物在束缚期之前或之后立即接受治疗，并在24小时后在EPM中进行测试。AP7对非应激大鼠的任何焦虑指标均无影响。在应激动物中，在EPM测试前注射该药物可增加进入开放臂的百分比。在束缚期之后立即给药时，AP7增加了在开放臂中停留的时间百分比，并且在相同臂中的进入百分比也有相同趋势。结果表明，干扰海马体NMDA受体会改变束缚应激在EPM中的致焦虑作用。
