Bleykasten-Grosshans C, Prior C, Potier S
Laboratoire de Microbiologie et Génétique, UPRES-A 7010, Université Louis Pasteur/CNRS, 28 rue Goethe, F-67083 Strasbourg, France.
Yeast. 2001 Jan 15;18(1):61-7. doi: 10.1002/1097-0061(200101)18:1<61::AID-YEA649>3.0.CO;2-Z.
We have isolated the Pichia sorbitophila LYS2 (PsLYS2) gene by complementation of a lys2 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 4197 bp and the deduced translation product shares a global identity of 66% and 58% to the Lys2 protein homologues of Candida albicans and S. cerevisiae, respectively. Analysis of PsLYS2 sequence suggests that, similarly to S. cerevisiae LYS2, it codes for a polypeptide having two separate enzymatic activities which reside in different domains of the protein, including an adenylate domain, an acyl-carrier site and a short-chain reductase domain. Several GCN4- and NIT2-binding motifs have been matched in the promotor sequence of PsLYS2. In addition, upstream of the sequenced PsLYS2 sequence, we have found the 3'-terminal half of a gene of same orientation encoding a RAD16-like protein, a genomic organization similar to that of C. albicans.
我们通过对酿酒酵母lys2突变体进行互补作用，分离出了嗜高渗毕赤酵母LYS2（PsLYS2）基因。测序的DNA片段包含一个4197 bp的推定开放阅读框，推导的翻译产物与白色念珠菌和酿酒酵母的Lys2蛋白同源物的整体一致性分别为66%和58%。对PsLYS2序列的分析表明，与酿酒酵母LYS2类似，它编码一种具有两种独立酶活性的多肽，这两种活性位于蛋白质的不同结构域，包括一个腺苷酸结构域、一个酰基载体位点和一个短链还原酶结构域。在PsLYS2的启动子序列中匹配到了几个GCN4和NIT2结合基序。此外，在测序的PsLYS2序列上游，我们发现了一个同向基因的3'末端一半，该基因编码一种RAD16样蛋白，其基因组组织与白色念珠菌相似。
