产气克雷伯菌膜囊泡中的柠檬酸盐摄取。
Citrate uptake in membrane vesicles of Klebsiella aerogenes.
作者信息
Johnson C L, Cha Y A, Stern J R
出版信息
J Bacteriol. 1975 Feb;121(2):682-7. doi: 10.1128/jb.121.2.682-687.1975.
In whole cells of Klebsiella aerogenes grown anaerobically on citrate as sole carbon source, citrate uptake is followed by rapid catabolism of the substrate via the inducible citrate fermentation pathway. Membrane vesicles prepared from such cells take up citrate but do not catabolize it. Vesicles process d-lactate dehydrogenase and the Na+-requiring oxalacetate decarboxylase. Citrate is taken up in the presence of Na+, and other monovalent cations, such as NH4+, Rb+, Cs+, or K+, do not substitute for Na+. Li+ appears to act synergistically with Na+. Citrate uptake is inhibited by N-2, cyanide, azide, sulfhydryl reagents, dinitrophenol, fluorcitrate, and hydroxycitrate.
在以柠檬酸盐作为唯一碳源进行厌氧生长的产气克雷伯菌的完整细胞中，柠檬酸盐被摄取后，底物会通过可诱导的柠檬酸盐发酵途径迅速分解代谢。从这类细胞制备的膜囊泡摄取柠檬酸盐，但不会对其进行分解代谢。膜囊泡含有d-乳酸脱氢酶和需要钠离子的草酰乙酸脱羧酶。柠檬酸盐在有钠离子存在的情况下被摄取，其他单价阳离子，如铵离子、铷离子、铯离子或钾离子，不能替代钠离子。锂离子似乎与钠离子起协同作用。柠檬酸盐的摄取受到N-2、氰化物、叠氮化物、巯基试剂、二硝基苯酚、氟柠檬酸和羟基柠檬酸的抑制。
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