Lawford H G, Williams G R
Biochem J. 1971 Jul;123(4):571-7. doi: 10.1042/bj1230571.
When cells of Pseudomonas are grown on citrate as the sole carbon source they oxidize citrate and isocitrate rapidly. Fluorocitrate inhibits the oxidation of citrate. Fluorocitrate-treated cells accumulate [6-(14)C]citrate, as shown by a rapid Millipore-filtration technique. In the absence of fluorocitrate most of the [6-(14)C]-citrate is lost in the form of (14)CO(2). The isolation of a pseudomonad characterized by its ability to grow on tricarballylate as a sole carbon source has facilitated the study of the tricarboxylate-carrier specificity. Cells grown on citrate will exchange radioactive citrate for unlabelled citrate or isocitrate but not for cis-aconitate, trans-aconitate or tricarballylate. Cells grown on tricarballylate will exchange radioactive citrate for unlabelled citrate, cis-aconitate or tricarballylate, but not for isocitrate or trans-aconitate. The properties of the exchange system involved are compared with those of the related system in mitochondria.
当假单胞菌细胞以柠檬酸盐作为唯一碳源生长时，它们会迅速氧化柠檬酸盐和异柠檬酸盐。氟代柠檬酸盐会抑制柠檬酸盐的氧化。通过快速微孔过滤技术表明，经氟代柠檬酸盐处理的细胞会积累[6-(14)C]柠檬酸盐。在没有氟代柠檬酸盐的情况下，大部分[6-(14)C]柠檬酸盐会以(14)CO(2)的形式损失。一种以能够以三羧基丙烷酸盐作为唯一碳源生长为特征的假单胞菌的分离，促进了对三羧酸载体特异性的研究。以柠檬酸盐生长的细胞会将放射性柠檬酸盐与未标记的柠檬酸盐或异柠檬酸盐进行交换，但不会与顺乌头酸盐、反乌头酸盐或三羧基丙烷酸盐进行交换。以三羧基丙烷酸盐生长的细胞会将放射性柠檬酸盐与未标记的柠檬酸盐、顺乌头酸盐或三羧基丙烷酸盐进行交换，但不会与异柠檬酸盐或反乌头酸盐进行交换。将所涉及的交换系统的特性与线粒体中相关系统的特性进行了比较。
