单细胞原始红藻梅氏嗜热蓝细菌中存在一个假定的线粒体ftsZ基因。

A putative mitochondrial ftsZ gene is present in the unicellular primitive red alga Cyanidioschyzon merolae.

作者信息

Takahara M, Takahashi H, Matsunaga S, Miyagishima S, Takano H, Sakai A, Kawano S, Kuroiwa T

机构信息

Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Japan.

出版信息

Mol Gen Genet. 2000 Nov;264(4):452-60. doi: 10.1007/s004380000307.

DOI:10.1007/s004380000307

PMID:11129049

Abstract

Two ftsZ homologues were isolated from the unicellular primitive red alga Cyanidioschyzon merolae (CmftsZ1 and CmftsZ2). Phylogenetic analysis revealed that CmftsZ1 is most closely related to the ftsZ genes of alpha-Proteobacteria, suggesting that it is a mitochondrial-type ftsZ gene, whereas CmftsZ2 is most closely related to the ftsZ genes of cyanobacteria, suggesting that it is a plastid-type ftsZ gene. Southern analysis indicates that CmftsZ1 and CmftsZ2 are both single-copy genes located on chromosome XIV in the C. merolae genome. Northern analysis revealed that both CmftsZ1 and CmftsZ2 are transcribed, and accumulate specifically before cell and organelle division. The results of Western analysis suggest that CmFtsZ1 is localized in mitochondria.

摘要

从单细胞原始红藻梅氏嗜热栖热菌（Cyanidioschyzon merolae）中分离出了两个ftsZ同源物（CmftsZ1和CmftsZ2）。系统发育分析表明，CmftsZ1与α-变形菌的ftsZ基因关系最为密切，这表明它是一种线粒体类型的ftsZ基因，而CmftsZ2与蓝细菌的ftsZ基因关系最为密切，这表明它是一种质体类型的ftsZ基因。Southern分析表明，CmftsZ1和CmftsZ2都是位于梅氏嗜热栖热菌基因组第十四条染色体上的单拷贝基因。Northern分析显示，CmftsZ1和CmftsZ2都能转录，并且在细胞和细胞器分裂之前特异性积累。Western分析结果表明，CmFtsZ1定位于线粒体中。