从单细胞原始红藻嗜硫加利藻中分离出的两种ftsZ基因。

Two types of ftsZ genes isolated from the unicellular primitive red alga Galdieria sulphuraria.

作者信息

Takahara M, Takahashi H, Matsunaga S, Sakai A, Kawano S, Kuroiwa T

机构信息

Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.

出版信息

Plant Cell Physiol. 1999 Aug;40(8):784-91. doi: 10.1093/oxfordjournals.pcp.a029606.

DOI:10.1093/oxfordjournals.pcp.a029606

PMID:10555302

Abstract

FtsZ plays a crucial role in bacterial cell division, and may be involved in plastid division in eukaryotes. To investigate the evolution of the dividing apparatus from prokaryotes to eukaryotes, the ftsZ genes were isolated from the unicellular primitive red alga Galdieria sulphuraria. Two ftsZ genes (GsftsZ1 and GsftsZ2) were isolated. This suggests that duplication and divergence of the ftsZ gene occurred in an early stage of plant evolution. A comparison of the FtsZs of G. sulphuraria and other organisms shows that FtsZ is highly and universally conserved among prokaryotes, primitive eukaryotic algae, and higher plants. The GsftsZ2 gene seems to contain an intron. Southern hybridization analysis of the G. sulphuraria chromosomes separated by CHEF revealed that each ftsZ gene and its flanking region may be duplicated.

摘要

FtsZ在细菌细胞分裂中起关键作用，并且可能参与真核生物中的质体分裂。为了研究从原核生物到真核生物的分裂装置的进化，从单细胞原始红藻嗜盐栖热袍菌中分离出ftsZ基因。分离出了两个ftsZ基因（GsftsZ1和GsftsZ2）。这表明ftsZ基因的复制和分化发生在植物进化的早期阶段。对嗜盐栖热袍菌和其他生物的FtsZs进行比较表明，FtsZ在原核生物、原始真核藻类和高等植物中高度且普遍保守。GsftsZ2基因似乎含有一个内含子。通过CHEF分离的嗜盐栖热袍菌染色体的Southern杂交分析表明，每个ftsZ基因及其侧翼区域可能已被复制。