Erwin R E, Randolph H E
J Dairy Sci. 1975 Jan;58(1):9-12. doi: 10.3168/jds.S0022-0302(75)84509-7.
The effect of mastitis on the milk fat globule membrane of milk samples from individual quarters was investigated. Wisconsin Mastitis Test positive (greater than 20mm) milk contained approximately 10% less milk fat globule membrane material than negative (smaller than 10mm) milk. The lipid portion of the milk fat globule membrane preparation from positive milk contained smaller amounts of phospholipids and larger amounts of cholesterol. Milk fat globule membrane preparations from positive milk had higher aldolase activity and lower xanthine oxidase activity. Dissociated membrane protein prepared from positive milk was resolved into five components by polyacrylamide gel electrophoresis while the membrane protein prepared from negative milk was resolved into three components.
研究了乳腺炎对来自各个乳腺区的牛奶样本中乳脂肪球膜的影响。威斯康星乳腺炎检测呈阳性(大于20毫米)的牛奶中乳脂肪球膜物质比呈阴性(小于10毫米)的牛奶约少10%。阳性牛奶的乳脂肪球膜制剂中的脂质部分含有较少量的磷脂和较大量的胆固醇。阳性牛奶的乳脂肪球膜制剂具有较高的醛缩酶活性和较低的黄嘌呤氧化酶活性。通过聚丙烯酰胺凝胶电泳,阳性牛奶制备的解离膜蛋白可分解为五个组分,而阴性牛奶制备的膜蛋白可分解为三个组分。