Gately C L, Gately M K, Mayer M M
J Immunol. 1975 Jan;114(1 Pt 1):10-6.
The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCi isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D-20,w of the mitogenic factor was estimated to be (9.9 plus and minus 0.2) times 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 plus and minus 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 plus and minus 0.006 g/ml by CsCi isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2.
通过凝胶过滤、蔗糖密度梯度超速离心和氯化铯等密度超速离心法，对来自豚鼠淋巴结细胞的促有丝分裂因子的分子尺寸进行了研究。促有丝分裂因子是通过用特异性抗原卵清蛋白刺激近交系豚鼠2或13品系的免疫淋巴结细胞产生的；促有丝分裂活性通过测量来自对卵清蛋白无免疫反应的同基因动物的靶淋巴结细胞中氚化胸腺嘧啶的掺入量来测定。通过在葡聚糖凝胶G - 100上进行凝胶过滤，促有丝分裂因子的D-20,w估计为(9.9±0.2)×10-7 cm2/秒，通过蔗糖密度梯度超速离心，表观S20,w估计为2.4±0.2 S。通过氯化铯等密度超速离心法测定浮力密度为1.324±0.006 g/ml，据此估计促有丝分裂因子的偏比容为0.71。根据这些数据，计算出促有丝分裂因子的分子量为20,000，摩擦比为1.2。
