从白血病T细胞中提取的一种可非特异性增强抗体反应的因子的纯化及特性

Purification and properties of a factor from leukaemic T cells which non-specifically enhances the antibody response.

作者信息

Mulcahy H L, Rubin A S, MacDonald A B

出版信息

Immunology. 1981 Jan;42(1):25-35.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1458217/

Abstract

A non-specific immunoenhancing factor from leukemia owl monkey peripheral T lymphocytes has been purified and partially characterized. Owl monkey enhancing factor, OMEF, is a glycoprotein with a molecular weight of 39,000 and an isoelectric point of pH 6.5. It acts at nanogram levels on day 2 of the in vitro immune response to enhance the number of plaque-forming cells produced to sheep red blood cells in a murine assay system but has no effect when added on day 0 to this system but has no effect when added on day 0 to this system. Antibodies to the purified factor have been produced and binding studies show that OMEF cross-reacts with other antisera raised to an enhancing factor produced by a mixture of human allogeneic lymphocytes but shows no cross-reactivity to a murine enhancing factor.

摘要

一种来自白血病夜猴外周血T淋巴细胞的非特异性免疫增强因子已被纯化并进行了部分特性鉴定。夜猴增强因子（OMEF）是一种糖蛋白，分子量为39000，等电点为pH 6.5。在体外免疫反应的第2天，它以纳克水平起作用，可增强小鼠检测系统中针对绵羊红细胞产生的空斑形成细胞的数量，但在第0天添加到该系统时则无作用。已制备了针对该纯化因子的抗体，结合研究表明，OMEF与针对人同种异体淋巴细胞混合物产生的增强因子所制备的其他抗血清发生交叉反应，但与小鼠增强因子无交叉反应。

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本文引用的文献

1

Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。

J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.

2

Acute lymphocytic leukemia in owl monkeys inoculated with herpesvirus saimiri.接种猴疱疹病毒的夜猴急性淋巴细胞白血病

Science. 1971 Mar 19;171(3976):1161-3. doi: 10.1126/science.171.3976.1161.

3

Specific heterologous enhancement of immune responses. VI. Partial purification of a nonspecific enhancing factor from supernates of allogeneically stimulated human lymphocyte cell lines.免疫反应的特异性异源增强。VI. 从同种异体刺激的人淋巴细胞系的上清液中部分纯化一种非特异性增强因子。

J Exp Med. 1974 Feb 1;139(2):439-44. doi: 10.1084/jem.139.2.439.

4

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。

Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.

5

Specific heterologous enhancement of immune responses. V. Isolation of a soluble enhancing factor from supernatants of specifically stimulated and allogeneically induced lymphoid cells.免疫反应的特异性异源增强。V. 从特异性刺激和同种异体诱导的淋巴细胞上清液中分离可溶性增强因子。

J Immunol. 1973 Nov;111(5):1314-25.

6

Specific heterologous enhancement of immune responses. 3. Partial characterization of supernatant material with enhancing activity.免疫反应的特异性异源增强。3. 对具有增强活性的上清液物质的部分特性分析。

J Immunol. 1972 Jun;108(6):1597-604.

7

T cell suppression in vitro. II. Nature of specific suppressive factor.体外T细胞抑制作用。II. 特异性抑制因子的性质。

Eur J Immunol. 1974 Oct;4(10):667-74. doi: 10.1002/eji.1830041006.

8

Activation of T and B lymphocytes in vitro. II. Biological and biochemical properties of an allogeneic effect factor (AEF) active in triggering specific B lymphocytes.体外T和B淋巴细胞的激活。II. 一种在触发特异性B淋巴细胞中起作用的同种异体效应因子（AEF）的生物学和生化特性。

J Exp Med. 1974 Jul 1;140(1):19-37. doi: 10.1084/jem.140.1.19.

9

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.人红细胞膜主要多肽的电泳分析。

Biochemistry. 1971 Jun 22;10(13):2606-17. doi: 10.1021/bi00789a030.

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Mode of action of a supernatant activity from T-cell cultures that nonspecifically stimulates the humoral immune response.来自T细胞培养物的一种能非特异性刺激体液免疫反应的上清液活性的作用方式。

Proc Natl Acad Sci U S A. 1974 Feb;71(2):512-6. doi: 10.1073/pnas.71.2.512.

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