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[D组链球菌的实验室诊断]

[Laboratory diagnosis of group D Streptococcus].

作者信息

Mihalcu F, Stănescu C

出版信息

Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1979 Jan-Mar;24(1):45-53.

PMID:111334
Abstract

After taxonomic listing of group D streptococci, the authors discuss the methodology of the bacteriologic diagnosis of infections produced by these bacteria, showing the way in which the pathological products are collected, the strains isolated and seeded. The strains are identified in two stages: (1) group identification by group antigen identification, resistance to oxacillin, development at +45 degrees, development on bilaesculin media; (2) species and variety identification by biochemical, physiologic and metabolic tests, by lysotyping and enterocinotyping. A description is given of the testing of sensitivity of the strain to antibiotics by determination of the minimal inhibitory concentration. The formulas of the media used for culture and identification are given.

摘要

在对D组链球菌进行分类列举后,作者讨论了这些细菌所致感染的细菌学诊断方法,展示了病理产物的采集方式、分离和接种菌株的方法。菌株分两个阶段进行鉴定:(1)通过群抗原鉴定、对苯唑西林的耐药性、45℃生长情况、在胆汁七叶苷培养基上的生长情况进行群鉴定;(2)通过生化、生理和代谢试验、溶菌酶分型和肠球菌分型进行种和变种鉴定。介绍了通过测定最低抑菌浓度来检测菌株对抗生素敏感性的方法。给出了用于培养和鉴定的培养基配方。

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