Volkoff H, Peter R E
Department of Biological Sciences, University of Alberta, Alb., T6G 2E9, Edmonton, Canada.
Brain Res. 2000 Dec 22;887(1):125-33. doi: 10.1016/s0006-8993(00)03001-8.
CART (cocaine- and amphetamine-regulated transcript) peptides are novel brain neuropeptides that have been shown to have a role in the control of feeding behavior in mammals. The effects of intracereboventricular (i.c.v.) administration of two CART fragments, CART (62-76) and CART (55-102) on feeding and behavioral activity of goldfish (Carassius auratus) were examined. Both CART peptide fragments inhibited food intake in goldfish. Co-injection of CART peptide and neuropeptide Y (NPY) reduced the increase in feeding caused by injection of NPY alone. CART (55-102) was more potent than CART (62-76). Co-treatment with CART (55-102) and orexin A inhibited the feeding response induced by orexin A alone. Feeding behavior was stimulated relative to saline controls following injection of CART (62-76) alone and co-injection of NPY and CART (62-76), but was not affected by treatment with CART (55-102) alone, NPY and CART (55-102) or orexin A and CART (55-102). Total behavioral activity was increased with NPY, orexin A, and both CART fragments alone as compared to saline controls, as well as in fish co-treated with NPY and CART (62-76) or NPY and CART (55-102) as compared to saline controls and NPY-treated fish. Tremors were seen in fish treated with CART (55-102) alone, and in fish co-treated with NPY and CART (55-102) and orexin A and CART (55-102). Co-treatment of the fish with NPY but not with orexin A significantly lowered the frequency of fish showing tremors as compared to fish treated with CART alone. These results indicate that CART peptides are involved in the regulatory pathways of feeding and behavioral activity in goldfish.
可卡因和苯丙胺调节转录肽（CART）是一类新型脑内神经肽，已被证明在哺乳动物摄食行为的控制中发挥作用。本研究检测了脑室内注射两种CART片段CART（62 - 76）和CART（55 - 102）对金鱼（Carassius auratus）摄食和行为活动的影响。两种CART肽片段均抑制金鱼的食物摄入。CART肽与神经肽Y（NPY）共同注射可减少单独注射NPY引起的摄食增加。CART（55 - 102）比CART（62 - 76）更有效。CART（55 - 102）与食欲素A共同处理可抑制单独注射食欲素A诱导的摄食反应。单独注射CART（62 - 76）以及NPY与CART（62 - 76）共同注射后，与生理盐水对照组相比，摄食行为受到刺激，但单独使用CART（55 - 102）、NPY与CART（55 - 102）或食欲素A与CART（55 - 102）处理对摄食行为无影响。与生理盐水对照组相比，单独使用NPY、食欲素A以及两种CART片段均可增加总行为活动，与生理盐水对照组和NPY处理组的鱼相比，NPY与CART（62 - 76）或NPY与CART（55 - 102）共同处理的鱼也有相同结果。单独使用CART（55 - 102）处理的鱼，以及NPY与CART（55 - 102）和食欲素A与CART（55 - 102）共同处理的鱼出现震颤。与单独使用CART处理的鱼相比，NPY共同处理而非食欲素A共同处理可显著降低出现震颤的鱼的频率。这些结果表明，CART肽参与了金鱼摄食和行为活动的调节途径。
