Departments of Biology and Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
Fish Physiol Biochem. 2013 Aug;39(4):979-91. doi: 10.1007/s10695-012-9756-4. Epub 2012 Dec 11.
In this study, the effects of peripheral (intraperitoneal) injections of D-amphetamine on feeding behavior were assessed in goldfish. Compared with the saline-injected group, amphetamine injections decreased food intake at doses ranging from 1 to 75 μg/g, but not 0.5 μg/g, but increased locomotor behavior, as indicated by the increased number of total feeding and non-feeding acts, at doses ranging from 2.5 to 25 μg/g. Amphetamine at high doses inhibited both food intake (at 25, 50 and 75 μg/g) and feeding behavior (at 75 μg/g). In the hypothalamus, the expression of orexin was down-regulated, and both CART 1 and CART 2 expressions were up-regulated in amphetamine-treated fish (50 μg/g) as compared to saline-injected fish, but amphetamine treatment had no effect on either hypothalamic TH or TRH expression. In the telencephalon, amphetamine treatment (50 μg/g) up-regulated CART 1, CART 2 and TH mRNA expressions but had no effect on either orexin or TRH. Our results suggest that, as in mammals, the orexin, CART and TH systems might be involved in amphetamine-induced feeding/locomotor responses in goldfish.
在这项研究中，评估了外周（腹腔内）注射 D-苯丙胺对金鱼摄食行为的影响。与生理盐水注射组相比，1 至 75μg/g 剂量的苯丙胺注射可减少食物摄入量，但 0.5μg/g 剂量的苯丙胺注射则不会，而 2.5 至 25μg/g 剂量的苯丙胺注射则会增加总摄食和非摄食行为的次数，从而增加运动行为。高剂量的苯丙胺可抑制摄食（25、50 和 75μg/g）和摄食行为（75μg/g）。与生理盐水注射组相比，在金鱼下丘脑，食欲素的表达下调，而 CART1 和 CART2 的表达上调（50μg/g），但苯丙胺处理对下丘脑 TH 或 TRH 的表达没有影响。在端脑，苯丙胺处理（50μg/g）可上调 CART1、CART2 和 TH mRNA 的表达，但对食欲素或 TRH 没有影响。我们的研究结果表明，与哺乳动物一样，食欲素、CART 和 TH 系统可能参与了苯丙胺诱导的金鱼摄食/运动反应。
