• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用荧光杂交探针通过实时聚合酶链反应快速检测肠出血性大肠杆菌

Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.

作者信息

Bellin T, Pulz M, Matussek A, Hempen H G, Gunzer F

机构信息

Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover, Germany.

出版信息

J Clin Microbiol. 2001 Jan;39(1):370-4. doi: 10.1128/JCM.39.1.370-374.2001.

DOI:10.1128/JCM.39.1.370-374.2001
PMID:11136804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87735/
Abstract

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

摘要

在本报告中，我们介绍了一种用于在LightCycler仪器上快速鉴定肠出血性大肠杆菌的PCR方案。在多重检测中，编码志贺毒素1和志贺毒素2的基因在单个反应毛细管中被检测。对多达32个样品进行完整分析大约需要45分钟。

相似文献

1
Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.使用荧光杂交探针通过实时聚合酶链反应快速检测肠出血性大肠杆菌
J Clin Microbiol. 2001 Jan;39(1):370-4. doi: 10.1128/JCM.39.1.370-374.2001.
2
Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction.在实时聚合酶链反应中使用SYBR Green检测肠出血性大肠杆菌的stx1、stx2和eae基因。
Diagn Microbiol Infect Dis. 2009 May;64(1):98-101. doi: 10.1016/j.diagmicrobio.2009.01.031.
3
Development of a multiplex real-time polymerase chain reaction for simultaneous detection of enterohemorrhagic Escherichia coli and enteropathogenic Escherichia coli strains.建立一种多重实时聚合酶链反应方法，用于同时检测肠出血性大肠杆菌和肠致病性大肠杆菌菌株。
Foodborne Pathog Dis. 2010 Jul;7(7):801-8. doi: 10.1089/fpd.2009.0457.
4
PCR detection of enterohemorrhagic Escherichia coli O145 in food by targeting genes in the E. coli O145 O-antigen gene cluster and the shiga toxin 1 and shiga toxin 2 genes.通过靶向大肠杆菌O145 O抗原基因簇中的基因以及志贺毒素1和志贺毒素2基因，对食品中的肠出血性大肠杆菌O145进行PCR检测。
Foodborne Pathog Dis. 2009 Jun;6(5):605-11. doi: 10.1089/fpd.2008.0254.
5
Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp.用于鉴定人致泻性大肠杆菌和志贺氏菌属的八重PCR及荧光毛细管电泳法
J Microbiol Methods. 2007 Feb;68(2):331-41. doi: 10.1016/j.mimet.2006.09.013. Epub 2006 Oct 31.
6
Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin.用于直接检测产生新型枯草杆菌蛋白酶细胞毒素的志贺毒素大肠杆菌菌株的多重聚合酶链反应
J Clin Microbiol. 2005 Jun;43(6):2944-7. doi: 10.1128/JCM.43.6.2944-2947.2005.
7
Evaluation of 5'-nuclease and hybridization probe assays for the detection of shiga toxin-producing Escherichia coli in human stools.评估5'-核酸酶和杂交探针检测法用于检测人粪便中产志贺毒素大肠杆菌的效果。
J Microbiol Methods. 2007 Sep;70(3):406-15. doi: 10.1016/j.mimet.2007.05.016. Epub 2007 Jun 2.
8
High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan.在日本屠宰场采集的牛粪便中发现大量产志贺毒素大肠杆菌。
FEMS Microbiol Lett. 2004 Sep 1;238(1):189-97. doi: 10.1016/j.femsle.2004.07.035.
9
Detection and quantification of Shiga toxin-encoding genes in sheep faeces by real-time PCR.通过实时荧光定量PCR检测和定量绵羊粪便中志贺毒素编码基因
Mol Cell Probes. 2005 Oct;19(5):363-70. doi: 10.1016/j.mcp.2005.06.006.
10
Use of ramification amplification assay for detection of Escherichia coli O157:H7 and other E. coli Shiga toxin-producing strains.使用分支扩增分析法检测大肠杆菌O157:H7及其他产志贺毒素大肠杆菌菌株。
J Clin Microbiol. 2005 Dec;43(12):6086-90. doi: 10.1128/JCM.43.12.6086-6090.2005.

引用本文的文献

1
Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.用于研究微生物-宿主相互作用的实时荧光定量PCR技术
Methods Microbiol. 2004;34:255-330. doi: 10.1016/S0580-9517(04)34010-9. Epub 2004 Dec 1.
2
Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.用于评估农业地表水和各种粪便中 Aliarcobacter faecis 和 Aliarcobacter lanthieri 流行情况的实时定量 PCR 检测方法的开发与应用。
BMC Microbiol. 2020 Jun 16;20(1):164. doi: 10.1186/s12866-020-01826-3.
3
Shiga Toxin-Producing Infection in Jönköping County, Sweden: Occurrence and Molecular Characteristics in Correlation With Clinical Symptoms and Duration of Shedding.瑞典延雪平省的产志贺毒素感染：与临床症状和排毒时间相关的发生情况及分子特征。
Front Cell Infect Microbiol. 2018 May 1;8:125. doi: 10.3389/fcimb.2018.00125. eCollection 2018.
4
Occurrence and quantification of Shiga toxin-producing from food matrices.食品基质中产志贺毒素菌的发生与定量分析。
Vet World. 2018 Feb;11(2):104-111. doi: 10.14202/vetworld.2018.104-111. Epub 2018 Feb 3.
5
Genetic makeup of Shiga toxin-producing Escherichia coli in relation to clinical symptoms and duration of shedding: a microarray analysis of isolates from Swedish children.产志贺毒素大肠杆菌的遗传构成与临床症状和排毒时间的关系：瑞典儿童分离株的微阵列分析。
Eur J Clin Microbiol Infect Dis. 2017 Aug;36(8):1433-1441. doi: 10.1007/s10096-017-2950-7. Epub 2017 Apr 19.
6
Shiga Toxin-Producing Escherichia coli in Diarrheal Stool of Swedish Children: Evaluation of Polymerase Chain Reaction Screening and Duration of Shiga Toxin Shedding.瑞典儿童腹泻粪便中产志贺毒素大肠杆菌：聚合酶链反应筛查及志贺毒素排泄持续时间的评估
J Pediatric Infect Dis Soc. 2016 Jun;5(2):147-51. doi: 10.1093/jpids/piv003. Epub 2015 Feb 17.
7
Quantitative analysis of pathogens in the lower respiratory tract of patients with chronic obstructive pulmonary disease.慢性阻塞性肺疾病患者下呼吸道病原体的定量分析
BMC Pulm Med. 2015 Aug 19;15:94. doi: 10.1186/s12890-015-0094-z.
8
Dientamoeba fragilis prevalence coincides with gastrointestinal symptoms in children less than 11 years old in Sweden.在瑞典，11岁以下儿童中脆弱双核阿米巴的流行率与胃肠道症状相符。
Eur J Clin Microbiol Infect Dis. 2015 Oct;34(10):1995-8. doi: 10.1007/s10096-015-2442-6. Epub 2015 Jul 15.
9
Design of an improved multiplex PCR method for diagnosis of enterohaemoraghic E.coli and enteropathogic E.coli pathotypes.一种用于诊断肠出血性大肠杆菌和肠道致病性大肠杆菌致病型的改进多重聚合酶链反应方法的设计
Gastroenterol Hepatol Bed Bench. 2012 Spring;5(2):106-11.
10
A qPCR assay to detect and quantify Shiga toxin-producing E. coli (STEC) in cattle and on farms: a potential predictive tool for STEC culture-positive farms.一种用于检测和定量牛及农场中产志贺毒素大肠杆菌（STEC）的qPCR检测方法：一种针对STEC培养阳性农场的潜在预测工具。
Toxins (Basel). 2014 Mar 27;6(4):1201-21. doi: 10.3390/toxins6041201.

本文引用的文献

1
Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR.通过多重聚合酶链反应检测动物粪便中志贺样毒素（stx1和stx2）、紧密黏附素（eaeA）和肠出血性大肠杆菌（EHEC）溶血素（EHEC hlyA）基因。
Appl Environ Microbiol. 1999 Feb;65(2):868-72. doi: 10.1128/AEM.65.2.868-872.1999.
2
Escherichia coli O157:H7.大肠杆菌O157:H7
Lancet. 1998 Oct 10;352(9135):1207-12. doi: 10.1016/S0140-6736(98)01267-7.
3
Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections.产志贺毒素大肠杆菌感染的发病机制与诊断
Clin Microbiol Rev. 1998 Jul;11(3):450-79. doi: 10.1128/CMR.11.3.450.
4
Escherichia coli O157 infection disaster in Japan, 1996.1996年日本大肠杆菌O157感染灾难
Eur J Emerg Med. 1997 Sep;4(3):165. doi: 10.1097/00063110-199709000-00009.
5
The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.罗氏LightCycler荧光定量PCR仪：一款具备快速温度控制功能的微量多样品荧光计。
Biotechniques. 1997 Jan;22(1):176-81. doi: 10.2144/97221pf02.
6
Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli.一起由受产志贺毒素大肠杆菌污染的干发酵香肠引发的溶血尿毒综合征暴发的分子微生物学调查。
J Clin Microbiol. 1996 Jul;34(7):1622-7. doi: 10.1128/JCM.34.7.1622-1627.1996.
7
Analysis of double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection using the monomeric dye SYBR green I.使用单体染料SYBR绿I通过激光诱导荧光检测的毛细管电泳法分析双链DNA。
Anal Biochem. 1995 Nov 1;231(2):359-65. doi: 10.1006/abio.1995.9986.
8
Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin.人源和猪源产志贺样毒素大肠杆菌O101菌株的克隆相关性
J Clin Microbiol. 1995 Dec;33(12):3174-8. doi: 10.1128/jcm.33.12.3174-3178.1995.
9
Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction.通过聚合酶链反应直接检测原代粪便培养物中的大肠杆菌志贺样毒素基因。
J Clin Microbiol. 1993 Nov;31(11):3063-7. doi: 10.1128/jcm.31.11.3063-3067.1993.
10
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.两端带有荧光染料的寡核苷酸提供了一种用于检测PCR产物和核酸杂交的淬灭探针系统。
PCR Methods Appl. 1995 Jun;4(6):357-62. doi: 10.1101/gr.4.6.357.