使用荧光杂交探针通过实时聚合酶链反应快速检测肠出血性大肠杆菌
Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.
作者信息
Bellin T, Pulz M, Matussek A, Hempen H G, Gunzer F
机构信息
Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover, Germany.
出版信息
J Clin Microbiol. 2001 Jan;39(1):370-4. doi: 10.1128/JCM.39.1.370-374.2001.
Abstract
In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.
摘要
在本报告中，我们介绍了一种用于在LightCycler仪器上快速鉴定肠出血性大肠杆菌的PCR方案。在多重检测中，编码志贺毒素1和志贺毒素2的基因在单个反应毛细管中被检测。对多达32个样品进行完整分析大约需要45分钟。
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