Paton Adrienne W, Paton James C
School of Molecular and Biomedical Science, University of Adelaide, Adelaide, S.A. 5005, Australia.
J Clin Microbiol. 2005 Jun;43(6):2944-7. doi: 10.1128/JCM.43.6.2944-2947.2005.
We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.
我们最近描述了一种由某些产志贺毒素大肠杆菌(STEC)菌株产生的新型AB5枯草杆菌蛋白酶细胞毒素。这种潜在的致命毒素可能导致人类严重的胃肠道和全身性疾病。在本研究中,我们开发了一种三价聚合酶链反应(PCR)检测方法,用于检测新型毒素A亚基基因subA以及stx1和stx2。该检测方法中使用的三对引物互不干扰,分别产生556、180和255 bp的扩增产物。该检测方法可用于确定STEC分离株的毒素基因型,以及直接检测原代粪便培养提取物中的毒素基因。