Shibuya K, Yoshioka T, Hashiba T, Satoh S
Laboratory of Bioadaptation, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan.
J Exp Bot. 2000 Dec;51(353):2067-73. doi: 10.1093/jexbot/51.353.2067.
Although the role of the gynoecium in natural senescence of the carnation flower has long been suggested, it has remained a matter of dispute because petal senescence in the cut carnation flower was not delayed by the removal of gynoecium. In this study, the gynoecium was snapped off by hand, in contrast to previous investigations where removal was achieved by forceps or scissors. The removal of the gynoecium by hand prevented the onset of ethylene production and prolonged the vase life of the flower, demonstrating a decisive role of the gynoecium in controlling natural senescence of the carnation flower. Abscisic acid (ABA) and indole-3-acetic acid (IAA), which induced ethylene production and accelerated petal senescence in carnation flowers, did not stimulate ethylene production in the flowers with gynoecia removed (-Gyn flowers). Application of 1-aminocyclopropane-1-carboxylate (ACC), the ethylene precursor, induced substantial ethylene production and petal wilting in the flowers with gynoecia left intact, but was less effective at stimulating ethylene production in the -Gyn flowers and negligible petal in-rolling was observed. Exogenous ethylene induced autocatalytic production of the gas and petal wilting in the -Gyn flowers. These results indicated that ethylene generated in the gynoecium triggers the onset of ethylene production in the petals of carnation during natural senescence.
尽管长期以来人们一直认为雌蕊在香石竹花自然衰老过程中发挥作用，但这一观点仍存在争议，因为去除雌蕊并不能延缓切花香石竹花瓣的衰老。在本研究中，与之前使用镊子或剪刀去除雌蕊的研究不同，此次是用手掐掉雌蕊。手动去除雌蕊可防止乙烯产生，并延长花朵的瓶插寿命，这表明雌蕊在控制香石竹花自然衰老过程中起决定性作用。脱落酸（ABA）和吲哚-3-乙酸（IAA）可诱导香石竹花产生乙烯并加速花瓣衰老，但在去除雌蕊的花朵（-Gyn花）中却不会刺激乙烯产生。施加乙烯前体1-氨基环丙烷-1-羧酸（ACC），在雌蕊完整的花朵中会诱导大量乙烯产生和花瓣枯萎，但在-Gyn花中刺激乙烯产生的效果较差，且观察到花瓣内卷现象可忽略不计。外源乙烯可诱导-Gyn花中气体的自催化产生和花瓣枯萎。这些结果表明，在自然衰老过程中，雌蕊产生的乙烯触发了香石竹花瓣中乙烯产生的起始。
