一种矮牵牛同源异型域亮氨酸拉链蛋白PhHD-Zip在花朵衰老过程中发挥重要作用。
A Petunia homeodomain-leucine zipper protein, PhHD-Zip, plays an important role in flower senescence.
作者信息
Chang Xiaoxiao, Donnelly Linda, Sun Daoyang, Rao Jingping, Reid Michael S, Jiang Cai-Zhong
机构信息
Department of Horticulture, Northwest A&F University, Yangling, Shaanxi, China ; Department of Plant Sciences, University of California Davis, Davis, California, United States of America.
Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Agricultural Research Service, Davis, California, United States of America.
出版信息
PLoS One. 2014 Feb 14;9(2):e88320. doi: 10.1371/journal.pone.0088320. eCollection 2014.
Flower senescence is initiated by developmental and environmental signals, and regulated by gene transcription. A homeodomain-leucine zipper transcription factor, PhHD-Zip, is up-regulated during petunia flower senescence. Virus-induced gene silencing of PhHD-Zip extended flower life by 20% both in unpollinated and pollinated flowers. Silencing PhHD-Zip also dramatically reduced ethylene production and the abundance of transcripts of genes involved in ethylene (ACS, ACO), and ABA (NCED) biosynthesis. Abundance of transcripts of senescence-related genes (SAG12, SAG29) was also dramatically reduced in the silenced flowers. Over-expression of PhHD-Zip accelerated petunia flower senescence. Furthermore, PhHD-Zip transcript abundance in petunia flowers was increased by application of hormones (ethylene, ABA) and abiotic stresses (dehydration, NaCl and cold). Our results suggest that PhHD-Zip plays an important role in regulating petunia flower senescence.
花朵衰老由发育和环境信号引发，并受基因转录调控。一种同源异型域-亮氨酸拉链转录因子PhHD-Zip在矮牵牛花朵衰老过程中上调。通过病毒诱导的基因沉默技术使PhHD-Zip沉默，未授粉和授粉花朵的寿命均延长了20%。沉默PhHD-Zip还显著降低了乙烯产量以及参与乙烯（ACS、ACO）和脱落酸（ABA，NCED）生物合成的基因转录本丰度。在沉默的花朵中，衰老相关基因（SAG12、SAG29）的转录本丰度也显著降低。过表达PhHD-Zip加速了矮牵牛花朵衰老。此外，施用激素（乙烯、ABA）和非生物胁迫（脱水、NaCl和低温）会增加矮牵牛花朵中PhHD-Zip的转录本丰度。我们的结果表明，PhHD-Zip在调控矮牵牛花朵衰老中起重要作用。
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