Baleja J D
Department of Biochemistry, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA.
Anal Biochem. 2001 Jan 1;288(1):1-15. doi: 10.1006/abio.2000.4815.
This Review covers the delineation and optimization of protein-lipid systems for study using solution-state NMR spectroscopy. The first half presents the necessary background for a membrane protein biochemist to initiate collaboration with an NMR spectroscopist. The second half provides guidelines for the spectroscopist on data collection, analysis for obtaining conformational information, and structure generation and assessment. Although the emphasis is on the study of peptides in detergent micelles, methods are outlined for larger membrane-associated proteins and for use of other solubilizing agents.
本综述涵盖了用于溶液态核磁共振波谱研究的蛋白质-脂质体系的描绘与优化。前半部分介绍了膜蛋白生物化学家与核磁共振波谱学家开展合作所需的背景知识。后半部分为波谱学家提供了数据收集、获取构象信息的分析以及结构生成与评估方面的指导方针。尽管重点是研究去污剂胶束中的肽，但也概述了针对更大的膜相关蛋白以及使用其他增溶剂的方法。
