Ge Y, Jippo T, Lee Y M, Adachi S, Kitamura Y
Department of Pathology, Osaka University Medical School, Suita, Japan.
Am J Pathol. 2001 Jan;158(1):281-92. doi: 10.1016/S0002-9440(10)63967-3.
Expression of mouse mast cell protease (mMCP) genes was examined with particular attention to the transactivation effect of mi transcription factor (MITF) and the expression differences between C57BL/6 (B6) and WB strains. We had reported the enhancing effect of MITF on the expression of mMCP-4, -5, and -6 genes in cultured mast cells (CMCs) of B6 strain, and in the present study we demonstrated the enhancing effect on the expression of mMCP-2 and -9 genes as well. The enhancing effect of MITF on the expression of mMCP-2, -4, -5, -6, and -9 genes was also detected in CMCs of the WB strain. The regulation of mMCP-2, -4, and -9 genes was localized to a specific promoter element (CANNTG) which was recognized and bound by MITF and which was conserved between the B6 and WB strains. On the other hand, the expression of mMCP-2, -4, and -9 genes was smaller in CMCs of the B6 strain when compared to their expression in CMCs of the WB strain. Although mMCP-5 is a chymase as mMCP-2, -4, and -9, and genes encoding all of the chymases are located on chromosome 14, the mMCP-5 gene was regulated in a manner distinct from mMCP-2, -4, and -9 genes.
研究了小鼠肥大细胞蛋白酶（mMCP）基因的表达，特别关注微小转录因子（MITF）的反式激活作用以及C57BL/6（B6）和WB品系之间的表达差异。我们曾报道过MITF对B6品系培养肥大细胞（CMC）中mMCP - 4、-5和-6基因表达的增强作用，在本研究中，我们还证明了其对mMCP - 2和-9基因表达也有增强作用。在WB品系的CMC中也检测到MITF对mMCP - 2、-4、-5、-6和-9基因表达的增强作用。mMCP - 2、-4和-9基因的调控定位于一个特定的启动子元件（CANNTG），该元件可被MITF识别并结合，且在B6和WB品系之间保守。另一方面，与WB品系的CMC相比，B6品系的CMC中mMCP - 2、-4和-9基因的表达较少。尽管mMCP - 5与mMCP - 2、-4和-9一样是一种糜蛋白酶，且所有糜蛋白酶的编码基因都位于14号染色体上，但mMCP - 5基因的调控方式与mMCP - 2、-4和-9基因不同。
