Mota M M, Pradel G, Vanderberg J P, Hafalla J C, Frevert U, Nussenzweig R S, Nussenzweig V, Rodríguez A
Department of Pathology, New York University School of Medicine, 341 East 25 Street, New York, NY 10010, USA.
Science. 2001 Jan 5;291(5501):141-4. doi: 10.1126/science.291.5501.141.
Intracellular bacteria and parasites typically invade host cells through the formation of an internalization vacuole around the invading pathogen. Plasmodium sporozoites, the infective stage of the malaria parasite transmitted by mosquitoes, have an alternative mechanism to enter cells. We observed breaching of the plasma membrane of the host cell followed by rapid repair. This mode of entry did not result in the formation of a vacuole around the sporozoite, and was followed by exit of the parasite from the host cell. Sporozoites traversed the cytosol of several cells before invading a hepatocyte by formation of a parasitophorous vacuole, in which they developed into the next infective stage. Sporozoite migration through several cells in the mammalian host appears to be essential for the completion of the life cycle.
细胞内细菌和寄生虫通常通过在入侵病原体周围形成内化液泡来侵入宿主细胞。疟原虫子孢子是由蚊子传播的疟疾寄生虫的感染阶段，它具有进入细胞的另一种机制。我们观察到宿主细胞质膜被突破，随后迅速修复。这种进入模式不会在子孢子周围形成液泡，随后寄生虫从宿主细胞中逸出。子孢子在穿过几个细胞的细胞质后，通过形成一个寄生泡侵入肝细胞，并在其中发育成下一个感染阶段。疟原虫子孢子在哺乳动物宿主体内穿过几个细胞的迁移过程似乎对其生命周期的完成至关重要。
